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EC number: 203-596-2 | CAS number: 108-58-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Principles of method if other than guideline:
- Most test fish had a somewhat larger size than the one recommended in the test guideline for the zebra fish and the loading (g fish / litre test medium) in the control and the test concentrations slightly exceeded the recommendation of the guideline (1 g/l). These deviations from the recommendations of the guideline are not regarded to be relevant to the results.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch number: 0900250090
Date of receipt: 2017-02-06
Expiry date: 2018-01-20
Purity: 98.87 %
Water solubility: 7.398 g/L at 20 °C - Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- Stability of test concentration/s during exposure: Examined several times by chemical analysis (HPLC) in the freshly prepared media and in the media after 24 hours of exposure.
Storage: Routinely, the samples were analysed immediately. Only in exceptional cases, they were stored overnight deep frozen and protected from light. - Vehicle:
- no
- Details on test solutions:
- Pre-treatment of test item and preparation of test item concentrations
100 mg of the test item were added each day to 1 litre of dilution water, treated for 60 seconds at 8000 rpm with an ultra turrax and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using a folded filter with a pore size of 7 - 12 µm and an aseptic filter (Sartobran sterile capsules) with a pore size of 0.45 µm + 0.2 µm.
To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 5000 mL. Finally 7 fish were given to all test item concentrations and the control.
Standard Solutions used for Calibration of the test item
Resorcindiacetate
22.8 mg of the test item were dissolved and made up with acetonitrile to the mark in a 100 mL volumetric flask to prepare a stock solution of 228 mg/L. Defined volumes of this stock solution were further diluted with acetonitrile / Millipore water (1/1) to obtain standard solutions in the range of 0.023 to 2.280 mg/L.
The concentration of the lowest used calibration solution was employed as the limit of quantification.
Resorcin
22.6 mg of the analytical standard were dissolved and made up with acetonitrile to the mark in a 100 mL volumetric flask to prepare a stock solution of 225,774 mg/L. The purity of the analytical standard was considered. Defined volumes of this stock solution were diluted with acetonitrile / Millipore water (15 %/85 %) to obtain standard solutions in the range of 0.023 to
2.258 mg/L.
The concentration of the lowest used calibration solution was employed as the limit of quantification.
These solutions were used to calibrate the HPLC-system.
Standard Solutions used for Verification of the Calibration of the test item, analytical standard and Blank Test
Resorcindiacetate
20.7 mg of the test item were dissolved and made up with acetonitrile to the mark in a 100 mL volumetric flask to prepare a stock solution of 207 mg/L. This solution was further diluted with acetonitrile / Millipore water to obtain a concentration of 0.828 mg/L. The daily verification of the calibration was performed with the diluted Standard Solution of Verification.
Resorcin
22.3 mg (respectively 24.6 mg) of the analytical standard were dissolved and made up with acetonitrile to the mark in a 100 mL volumetric flask to prepare a stock solution of 222.777 mg/L (respectively 245.754 mg/L). The purity of the analytical standard was considered. This solution was further diluted with acetonitrile / Millipore water to obtain a concentration of 0.8911 mg/L (respectively 0.9830 mg/L). The daily verification of the calibration was performed with the diluted Standard Solution of Verification.
The mean recovery rate was calculated.
Acetonitrile / Millipore water (1/1) without the test item was analysed (analytical blank). - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Source: Interaquaristik.de Shop (Germany)
Date supplied: 2017-07-04
Acclimatisation: Stock held since 2017-07-04 and acclimatised to the test conditions since then.
Temperature: 20 - 24 °C
Dissolved oxygen: > 5 mg/L
Feeding: Commercial fish food, daily. Feeding discontinued 24 h prior to test start.
Mortalities during acclimatisation period: < 5 %
Medication: none
Mean standard length (n = 42): 3.84 cm (S.D. = 0.24 cm) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 13.6 °dH (= 243 mg/L CaCO3)
- Test temperature:
- 22.1 to 22.8 °C
- pH:
- 7.3 - 7.7
- Dissolved oxygen:
- 8.9 - 7.9 mg/L (100 - 90% saturation)
- Nominal and measured concentrations:
- 0.31, 0.63, 1.3, 2.5 and 5 mg/L (nominal)
- Details on test conditions:
- Exposure conditions
Test vessels: glass aquaria holding 5 L of test media covered by glass plates
Experimental design: 5 test concentrations plus 1 control
7 fish per test concentration
no feeding during the exposure period
semi-static system
Method of initiation: fish were placed in prepared media
Loading: 1.25 g body weight (wet weight) per litre
Photoperiod: 16 h light: 8 h dark
Temperature: 22.1 to 22.8 °C
Aeration: gentle aeration via narrow glass tubes
Method of administration: stock solution
Medium renewal: daily
Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.588 - < 1.212 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 2.604 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.588 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.185 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC100
- Effect conc.:
- 2.604 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.588 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.212 - < 2.604 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 2.604 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.588 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.729 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % confidence limit: 1.380 - 2.245
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- 5.413 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % confidence limit: 1.380 - 2.245
- Duration:
- 24 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1.171 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % confidence limit: 1.380 - 2.245
- Details on results:
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed.
All results are expressed in terms of geometric mean measured concentrations calculated on base of the sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin. Because of a lacking dose/response relationship the LC 50 values at 48 h and 96 hours could not be determined by probit analysis.
Effective concentrations calculated as sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin ranged from 106.5 % to 128.6 % of nominal values in the freshly prepared media and from 55.0 % to 121.8 % of nominal values in the media after 24 hours of exposure according to the semi-static test conditions. - Reported statistics and error estimates:
- The LC 50 was calculated by probit analysis using the statistics programme ToxRatPro Version 2.10 (released 2010-09-10).
- Sublethal observations / clinical signs:
Results of the probit analysis
Results of the probit analysis: Selected effective concentrations (LCx) of the test item and their 95%- and 99%-confidence limits (according to Fieller`s theorem).
Parameter LC10 LC20 LC50 Value [mg/L] 1.175 1.342 1.729 lower 95%-cl 0.764 0.957 1.380 upper 95%-cl 1.460 1.654 2.245 lower 95%-cl 0.690 0.878 1.278 upper 95%-cl 1.616 1.803 2.424 Slope function after Litchfield and Wilcoxon: 1.352
Results of the probit analysis: Selected effective concentrations (LCx) of the test item and their 95%- and 99%-confidence limits (according to Fieller`s theorem).
Parameter LC10 LC20 LC50 Value [mg/L] 1.012 1.068 n.d. lower 95%-cl n.d. n.d. n.d. upper 95%-cl n.d. n.d. n.d. lower 95%-cl n.d. n.d. n.d. upper 95%-cl n.d. n.d. n.d. n.d.: not determined due to mathematical reasons or inappropriate data
Slope function after Litchfield and Wilcoxon: 1,131
- Validity criteria fulfilled:
- yes
- Remarks:
- The mortality in the controls did not exceed 10 % by the end of the test. The dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period. The pH did not vary by more than 1 unit.
- Conclusions:
- Acute toxicity of Resorcindiacetat to Danio rerio under semi-static conditions shows a LC50 >0.588 mg/L and <1.212 mg/L after 96 hours.
- Executive summary:
A study was performed to assess the acute toxicity of Resorcindiacetat to Danio rerio under semi-static conditions with daily renewal of test solutions.
The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
Groups of seven fish of the recommended size were exposed to a range of concentrations, nominally 0.31, 0.63, 1.3, 2.5 and 5 mg/L of Resorcindiacetat dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax, a magnetic stirrer, a folded filter and an aseptic filter.
Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The following values were determined:
Time [h] LC50 [mg/L] Geometric mean LC0/LC100 [mg/L] 2 -- 3.979 24 1.729 48 > 1.212 < 2.604 72 1.185 96 > 0.588 < 1.212 All results are expressed in terms of geometric mean measured concentrations calculated on base of the sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin. Because of a lacking dose/response relationship the LC 50 values at 48 h and 96 hours could not be determined by probit analysis.
Effective concentrations calculated as sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin ranged from 106.5 % to 128.6 % of nominal values in the freshly prepared media and from 55.0 % to 121.8 % of nominal values in the media after 24 hours of exposure according to the semi-static test conditions.
The hardness of the dilution water used was 13.6 °dH (= 243 mg/L CaCO3).
Reference
Description of key information
A study was performed to assess the acute toxicity of Resorcindiacetat to Danio rerio under semi-static conditions with daily renewal of test solutions.
The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
Groups of seven fish of the recommended size were exposed to a range of concentrations, nominally 0.31, 0.63, 1.3, 2.5 and 5 mg/L of Resorcindiacetat dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax, a magnetic stirrer, a folded filter and an aseptic filter.
Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The following values were determined:
Time [h] | LC50 [mg/L] | Geometric mean LC0/LC100 [mg/L] |
2 | -- | 3.979 |
24 | 1.729 | |
48 | > 1.212 < 2.604 | |
72 | 1.185 | |
96 | > 0.588 < 1.212 |
All results are expressed in terms of geometric mean measured concentrations calculated on base of the sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin. Because of a lacking dose/response relationship the LC 50 values at 48 h and 96 hours could not be determined by probit analysis.
Effective concentrations calculated as sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin ranged from 106.5 % to 128.6 % of nominal values in the freshly prepared media and from 55.0 % to 121.8 % of nominal values in the media after 24 hours of exposure according to the semi-static test conditions.
The hardness of the dilution water used was 13.6 °dH (= 243 mg/L CaCO3).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.588 mg/L
Additional information
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