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EC number: 231-327-9 | CAS number: 7493-57-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 January 2017 - 8 March 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2006 (Annex 5 corrected 28 July 2011)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 2008 (amended by EC No. 2016/266 )
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult items and mixtures, OECD series on testing and assessment number 23
- Version / remarks:
- December 14, 2000
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Solubility in water: 86 mg/L at 24°C
- Vapour pressure: 0.60 Pa at 24°C - Analytical monitoring:
- yes
- Details on sampling:
- Single samples for analysis were taken from all test concentrations and the control.
Frequency at t=0 h, t=24 h and t=72 h
Volume 3.0 mL
Storage Samples were stored in a freezer (≤-15ºC) until analysis.
At the end of the exposure period, the replicates with algae were pooled at each concentration before sampling.
Compliance with the quality criteria regarding maintenance of actual concentrations was checked by running an additional test vessel at 10% (v/v) of the saturated solution prepared at a loading rate of 100 mg/L, but without algae, and samples for analysis were taken at the start, after 24 hours of exposure and at the end of the test period. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: preparation of a saturated solution (direct addition to test medium) - A loading rate of 100 mg/L was stirred overnight on a magnetic stirring device to accelerate the dissolution of the test item in medium. The obtained mixture was allowed to settle for 30 minutes. The resulting aqueous Saturated Solution (SS) was siphoned off and used as the highest test concentration. Lower test concentrations were prepared by subsequent dilutions of the SS in test medium. All test solutions were clear and colorless at the end of the preparation procedure.
After preparation, volumes of 50 mL were added to each replicate of the respective test concentration. Subsequently, 1 mL of an algal suspension was added to each replicate providing a cell density of 10^4 cells/mL.
- Controls: test medium without test item or other additives
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: NIVA CHL 1
- Source: In-house laboratory culture
- Age of inoculum (at test initiation): 3 days before the start of the test, cells from the algal stock culture were inoculated in culture medium (M2) at a cell density of 1 x 10^4 cells/mL.
- Method of cultivation: Algae stock cultures were started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 21-24°C. Stock culture medium = M1 (according to the NPR 6505 (“Nederlandse Praktijk Richtlijn no. 6505”) formulated using Milli-RO water (tap-water purified by reverse osmosis)
ACCLIMATION
- Acclimation period: yes (3 days)
- Culturing media and conditions (same as test or not): yes, pre-culture medium and test medium are the same (M2, according to OECD 201), as are pre-culture and test conditions
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg/L (as CaCO3)
- Test temperature:
- 22-23°C
- pH:
- 8.0-8.1
- Nominal and measured concentrations:
- Nominal test concentrations: 1.0, 3.2, 10, 32 and 100% (v/v) of a saturated solution prepared at a loading rate of 100 mg/L.
At the start of the test, the actual test concentrations were in agreement with the nominal concentrations, i.e., were 0.87, 2.8, 8.6, 27.1 and 89.1 mg/L in 1.0, 3.2, 10, 32 and 100% (v/v) of the saturated solution.
At the end of the test, these measured concentrations had decreased to 15-53% of the initial concentrations; i.e. 0.13, 1.02, 4.13, 14.4 and 31.0 mg/L in 1.0, 3.2, 10, 32 and 100% (v/v) of the saturated solution.
Based on these results, the Time Weighted Average (TWA) exposure concentrations were calculated and used to determine the effect parameters and these were: 0.43, 1.7, 6.0, 18 and 51 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass, capped
- Fill volume: 50 mL
- Aeration: no
- Initial cells density: 1 x 10^4 cells/mL
- Control end cells density: 93.2 x 10^4 cells/mL (mean, n=6)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- Other: 1 or 2 replicates of each test concentration without algae. 1 extra replicate of each test concentration for sampling purposes after 24 hours of exposure.
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-RO water (tap-water purified by reverse osmosis)
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature - continuously in a temperature control vessel; pH - at the beginning and at the end of the test for all test concentrations and the control
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: TLD-lamps with a light intensity within the range of 84 to 87 µE/m^2/s.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Determination of cell concentrations: At the beginning of the test, cells were counted using a microscope and a counting chamber. Thereafter, cell densities were determined by spectrophotometric measurement of samples at 680 nm using a spectrophotometer with cuvettes (path length = 10 mm). Algal medium was used as blank and the extra replicates as background for the treated solutions.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 3.2
RANGE FINDING STUDY
- Test concentrations: 0.1, 1.0, 10 and 100% (v/v) of a saturated solution prepared at a loading rate of 100 mg/L
- Results used to determine the conditions for the definitive study: yes; expected EC50 for both growth rate inhibition and yield inhibition was between 10% and 100% (v/v) of a saturated solution prepared at a loading rate of 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (February 2017)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.4 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% C.I. 7.4-13 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.8 g/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% C.I. 5.3-6.0 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.7 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities: no - Microscopic observations at the end of the test revealed a normal and healthy appearance of the algal cells exposed to a TWA concentration of 6.0 mg/L when compared to the control.
- Any stimulation of growth found in any treatment: yes - at the two lowest test concentrations
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Other: After 24 hours, the measured concentrations decreased to 63-79% of the initial concentrations. At the end of the test, the measured concentrations decreased to 15-53% of the initial concentrations. The concentrations measured in the samples without algae were comparable, thus it is unlikely that the substance was lost due to sorption by the algal biomass.
- Effect concentrations exceeding solubility of substance in test medium: no
pH and temperature were within the limits prescribed in the OECD guideline. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- 72h-ErC50: 1.2 mg/L (95% C.I. 1.1-1.2 mg/L)
- Other: The ErC50 value for the positive control fell into the historical ranges for growth rate inhibition (between 0.82 and 2.3 mg/L). - Reported statistics and error estimates:
- An effect was considered to be significant if statistical analysis of the data obtained for the test concentrations compared with those obtained in the negative control revealed significant inhibition of growth rate or inhibition of yield (Williams Multiple Sequential t-test Procedure, α=0.05, one-sided, smaller).
Since stimulation rather than inhibition of growth rate was observed at the two lowest concentrations and complete inhibition at the two highest test concentrations, the lowest and the highest test group were removed from the statistical determination of the NOEC.
Due to mathematical reasons (The requirements for the parametric test (i.e. normality of data and homogeneity of variance) were not fulfilled and the replication was not sufficient for a non-parametric test), statistical analysis would otherwise not have been possible to be performed for the data gathered after 72 hours of exposure to determine the NOEC.
The EC10 was still calculated as well as an equivalent alternative.
Calculation of ECx values was based on probit analysis using linear max. likelihood regression with the percentages of growth rate inhibition versus the logarithms of the corresponding TWA exposure concentrations of the test item.
The calculations were performed with ToxRat Professional v. 3.2.1 (ToxRat Solutions® GmbH, Germany). - Validity criteria fulfilled:
- yes
- Remarks:
- see 'Overall remarks' field
- Conclusions:
- The ErC50, ErC10 and NOEC were 8.4, 5.8 and 1.7 mg/L, respectively.
- Executive summary:
A study was performed to assess the effect of the substance on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD TG No 201. The test was also performed under GLP and assigned a Klimisch 1 rating. Test solutions were prepared at a loading rate of 100 mg/L, resulting in an aqueous Saturated Solution (SS) that was used as the highest test concentration. Lower test concentrations (1.0, 3.2, 10 and 32% (v/v) of the SS) were prepared by subsequent dilutions of the 100% SS in test medium. Pseudokirchneriella subcapitatawas exposed for 72 hours (three replicate flasks per concentration, six replicate flasks per control). Concentrations were analyzed at t = 0, 24 and 72 h. The initial measured concentration in the 100% saturated solution was 89% of nominal, and in dilutions from the 100% saturated solution in the range 85 -88% of nominal. At the end of the test, the measured concentrations had decreased to 15-53% of the initial concentrations. The concentrations measured in the samples without algae were comparable, thus it is unlikely that the substance was lost due to sorption by the algal biomass. Since test concentrations gradually decreased during the test period, time-weighted average measured concentrations were calculated: 0.43, 1.7, 6.0, 18 and 51 mg/L, corresponding with 1.0%, 3.2%, 10%, 32% (v/v) and 100% of the SS prepared at a loading rate of 100 mg/L. Statistically significant inhibition of growth rate (and yield) was found at test concentrations of 6.0 mg/L and higher. The ErC50, ErC10 and NOEC were 8.4, 5.8 and 1.7 mg/L, respectively, based on time-weighted average measured concentrations.
Reference
Final Test: Test Samples
Time of sampling |
Percentage of SS1 |
Analyzed concentration2 |
Relative to initial |
0 |
0 |
< LOQ |
|
|
1.0 |
0.8724 |
|
|
3.2 |
2.83 |
|
|
10 |
8.57 |
|
|
103 |
8.71 |
|
|
32 |
27.1 |
|
|
100 |
89.1 |
|
24 |
0 |
< LOQ |
n.a. |
|
1.0 |
0.6034 |
69 |
|
3.2 |
1.93 |
68 |
|
10 |
6.62 |
77 |
|
103 |
6.91 |
79 |
|
32 |
17.0 |
63 |
|
100 |
55.9 |
63 |
72 |
0 |
< LOQ |
n.a. |
|
1.0 |
0.1314 |
15 |
|
3.2 |
1.024 |
36 |
|
10 |
4.13 |
48 |
|
103 |
4.04 |
46 |
|
32 |
14.4 |
53 |
|
100 |
31.0 |
35 |
1 Percentage of a saturated solution (SS) prepared at a loading rate of 100 mg/L.
2 Samples were stored in the freezer (≤ -15°C) until the day of analysis.
3 Without algae.
4 Content calculated after correction of the test item response with the mean peak area of the background of the blank QC samples.
< LOQ Below the limit of quantification (LOQ) at 0.1 mg/L.
n.a. Not applicable.
Based on these results, the Time Weighted Average (TWA) exposure concentrations were calculated and used to determine the effect parameters.
Measured concentrations versus nominal concentrations
Hyacinth Body # 3 |
Measured concentration (mg/L) |
TWA (mg/L) |
||
t=0h |
t=24h |
t=72 h |
||
1.0 |
0.872 |
0.603 |
0.131 |
0.43 |
3.2 |
2.83 |
1.93 |
1.02 |
1.7 |
10 |
8.57 |
6.62 |
4.13 |
6.0 |
101 |
8.71 |
6.91 |
4.04 |
6.1 |
32 |
27.1 |
17.0 |
14.4 |
18 |
100 |
89.1 |
55.9 |
31.0 |
51 |
1without algae
Biological results
A stimulation of growth rates was observed at the two lowest test concentrations during the 72-hour test period, whereas the growth rate of algae exposed to TWA concentrations of 6.0 mg/L and higher were increasingly reduced.
Statistically significant inhibition of growth rate and yield was found at test concentrations of 6.0 mg/L and higher.
Percentage inhibition of growth rate (total test period) during the final test
Hyacinth Body # 3 |
Mean |
Std. Dev. |
n |
%Inhibition |
Control |
1.510 |
0.0334 |
6 |
|
0.43 |
1.565 |
0.0212 |
3 |
-3.61 |
1.7 |
1.576 |
0.0242 |
3 |
-4.4 |
6.0 |
1.320 |
0.1017 |
3 |
12.6* |
18 |
0.008 |
0.0136 |
3 |
99.5* |
51 |
0.012 |
0.0209 |
3 |
99.21 |
1Test group not used in statistical determination of the NOEC.
* effect was statistically significant
Percentage inhibition of growth rate at different time intervals during the final test
Hyacinth Body # 3 |
n |
0 – 24 h |
24 – 48 h |
48 – 72h |
|||
Mean |
%Inhibition |
Mean |
%Inhibition |
Mean |
%Inhibition |
||
Control |
6 |
1.032 |
|
1.878 |
|
1.620 |
|
0.43 |
3 |
1.288 |
-24.8 |
1.788 |
4.8 |
1.619 |
0.0 |
1.7 |
3 |
1.558 |
-51.0 |
1.543 |
17.8 |
1.628 |
-0.5 |
6.0 |
3 |
0.757 |
26.7 |
1.637 |
12.9 |
1.567 |
3.3 |
18 |
3 |
0.627 |
39.2 |
-0.465 |
124.8 |
-0.139 |
108.6 |
51 |
3 |
0.012 |
98.8 |
0.187 |
90.0 |
-0.163 |
110.1 |
Description of key information
A study was performed to assess the effect of the substance on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD TG No 201. The test was also performed under GLP and assigned a Klimisch 1 rating. Test solutions were prepared at a loading rate of 100 mg/L, resulting in an aqueous Saturated Solution (SS) that was used as the highest test concentration. Lower test concentrations (1.0, 3.2, 10 and 32% (v/v) of the SS) were prepared by subsequent dilutions of the 100% SS in test medium. Pseudokirchneriella subcapitata was exposed for 72 hours (three replicate flasks per concentration, six replicate flasks per control). Concentrations were analyzed at t = 0, 24 and 72 h. The initial measured concentration in the 100% saturated solution was 89% of nominal, and in dilutions from the 100% saturated solution in the range 85 -88% of nominal. At the end of the test, the measured concentrations had decreased to 15-53% of the initial concentrations. The concentrations measured in the samples without algae were comparable, thus it is unlikely that the substance was lost due to sorption by the algal biomass. Since test concentrations gradually decreased during the test period, time-weighted average measured concentrations were calculated: 0.43, 1.7, 6.0, 18 and 51 mg/L, corresponding with 1.0%, 3.2%, 10%, 32% (v/v) and 100% of the SS prepared at a loading rate of 100 mg/L. Statistically significant inhibition of growth rate (and yield) was found at test concentrations of 6.0 mg/L and higher. The ErC50, ErC10 and NOEC were 8.4, 5.8 and 1.7 mg/L, respectively, based on time-weighted average measured concentrations.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8.4 mg/L
- EC10 or NOEC for freshwater algae:
- 5.8 mg/L
Additional information
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