Octanethioic acid, S-[3-(triethoxysilyl)propyl] ester, reaction products with 2-methyl-1,3-propanediol and 3-(triethoxysilyl)-1-propanethiol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23.02.2007 - 27.02.2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

OECD No.203, 1992

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

according to guideline

Guideline:

other: OECD Series on Testing and Assessment, No. 23, “Guidance Document on Aquatic Toxicitiy Testing of Difficult Substances and Mixtures", December 15, 2000

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Duplicate samples from the freshly prepared test media of the only test concentration and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions, respectively the maintenance of the test item concentrations during the test period, duplicate samples were taken in duplicates out of the only test medium and the control at the end of the test after 96 hours of exposure.
The concentration of the test item Y-15821 were analysed in the duplicate test media samples of the test concentration of nominal 100 mg test item/L from both sampling times (0 and 96 hours). From the control samples only one of the duplicate samples was analysed from each of

Vehicle:

no

Details on test solutions:

100 mg test item/L. Additionally, a control was tested in parallel (test water without addition of the test item).
A limit test was performed in accordance with the Commission Directive 92/69/EEC to demonstrate that the test item has no toxic effect on the test fish up to the concentration of nominal 100 mg test item/L.
The medium of the only test concentration of nominal 100 mg/L was prepared by dissolving 3x500 mg test item into 3x50000 mL test water by intense stirring for 24 hours.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Species: Rainbow trout (Oncorhynchus mykiss)
Age and Size: Juveniles
The mean body length of the fish* in the test was 4.34 +/- 0.19 cm (Mean +/- SD), the mean body wet weight 0.66 +/- 0.08 g (Mean +/- SD).
* 10 fish from the test fish batch were measured during the course of the test
Sex: Male and female
Origin: The test fish were obtained from Forellenzuchtbetrieb Tautenhahn, 98646 Trostadt, Germany
Holding Conditions: In accordance with the test guidelines the fish were held in test water in the laboratories of IBACON for at least 12 days prior to the start of the test without any medication. During holding until one day before test start the fish
were fed with a commercial fish diet for Rainbow trout. During the last 7 days prior to the start of the test no fish died in the test fish batch and all fish were healthy
Acclimatisation: For at least 12 days before the start of the test the fish were acclimated to the test water and test temperature.

Test type:

static

Water media type:

other: reconstituted water

Limit test:

yes

Total exposure duration:

96 h

Hardness:

250 mg CaCO3/L

Test temperature:

14 - 15 °C

pH:

6.5 - 8.5

Dissolved oxygen:

at least 60% of the air saturation value

Nominal and measured concentrations:

Nominal: 100 mg test item/L
Mean measured: 81 mg test item/L

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item Y-15821 to Rainbow Trout was assessed in a static limit test. The 96-hour LC50 value was > 100 mg test item/L.

Executive summary:

The 96-hour LC50 value was > 100 mg test item/L. No mortality or signs of toxicity were abserved in any treated fish at 100mg test item/L.

Description of key information

The 96-hour LC50 value was > 100 mg test item/L. No mortality or signs of toxicity were abserved in any treated fish at 100 mg test item/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

100 mg/L