N-(phenylsulphonyl)benzenesulphonamide

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for N-(benzenesulfonyl)benzenesulfonamide (2618-96-4). The EC50 value was estimated to be 222.387 mg/l when N-(benzenesulfonyl)benzenesulfonamide exposed to Daphnia magna for 48hrs. Based on this value it can be concluded that the substance N-(benzenesulfonyl)benzenesulfonamideis considered to be not toxic to aquatic environment as per the criteria mentioned in CLP regulation. 

Toxicity to aquatic algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for N-(benzenesulfonyl) benzenesulfonamide (2618-96-4). The EC50 value was estimated to be 112.831 mg/l when N-(benzenesulfonyl)benzenesulfonamide exposed to Desmodesmus subspicatus for 72hrs. Based on this value it can be concluded that the substance N-(benzenesulfonyl) benzenesulfonamide is considered to be not toxic to aquatic environment as per the criteria mentioned in CLP regulation. 

 

Additional information

Summarized result of toxicity of N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) on the growth and other activity of aquatic invertebrates, algae and cyanobacteria by considering the data for target as well as RA chemical which was selected on the basis of structural similarity and log Kow as the primary descriptor are as follows:   

Short term toxicity to aquatic invertebrates:

Various predicted data for the target compound N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) and supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor and on the basis of structural and functional similarity were reviewed for the toxicity on the invertebrates end point which are summarized as below: 

In a prediction done by SSS (2017), Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for N-(benzenesulfonyl)benzenesulfonamide (2618-96-4). The EC50 value was estimated to be 222.387 mg/l when N-(benzenesulfonyl)benzenesulfonamide exposed to Daphnia magna for 48hrs. Based on this value it can be concluded that the substance N-(benzenesulfonyl)benzenesulfonamideis considered to be not toxic to aquatic environment as per the criteria mentioned in CLP regulation. 

 

In second prediction using the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 721.924 mg/l for N-(benzenesulfonyl)benzenesulfonamide in 48 hrs. Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.  

 

Similarly in a supporting weight of evidence study from Chemosphere, 2009, for read across chemical (59-40-5) study were to determine the toxicity of a chemical. Aim of the study was to determine the effect of chemical 4-amino-N-(quinoxalin-2-yl) benzene-1-sulfonamide (Sulfaquinoxaline) when exposed with the test organism daphnia magna. Test was performed according to the OECD Guideline 202 ‘Daphnia sp., Acute Immobilisation Test’. Test conducted on the nominal 50-500 mg/l concentration. Juvenile daphnids <24-h old were used as a test organism. Chemical was solubilized in ADaM medium. The organisms were derived from a single clone of D. magna Straus cultured and maintained in ADaM medium. Daphnia fed on Scenedesmus dimorphus every other day having 8 × 105cells mL/l. Four groups of 5 young daphnids were exposed to each concentration tested or used as controls. EC50 with 95% confidence limits was estimated by probit analysis using SPSS 16.0 software or by Spearman–Karber method. Based on the immobility of daphnia magna Straus due to the chemical contact 4-amino-N-(quinoxalin-2-yl)benzene-1-sulfonamide (Sulfaquinoxaline) for 48 hrs, the EC50 was 131 mg/l with 95 % CI was 119-143 mg/l. Thus chemical was consider as nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

Similarly fourth study was conducted on the read across chemical selected on the basis of structure similarity (68-35-9) from Chemosphere, 2009, Study was conducted to determine the effect of chemical 4-amino-N-pyrimidin-2-ylbenzenesulfonamide (Sulfadiazine) when exposed with the test organism daphnia magna. Test was performed according to the OECD Guideline 202 ‘Daphnia sp., Acute Immobilisation Test’. Test conducted on the nominal 50-500 mg/l concentration. Juvenile daphnids <24-h old were used as a test organism. Chemical was solubilized in ADaM medium. The organisms were derived from a single clone of D. magna Straus cultured and maintained in ADaM medium. Daphnia fed on Scenedesmus dimorphus every other day having 8 × 105cells mL/l. Four groups of 5 young daphnids were exposed to each concentration tested or used as controls. EC50 with 95% confidence limits was estimated by probit analysis using SPSS 16.0 software or by Spearman–Karber method. Based on the immobility of daphnia magna Straus due to the chemical contact 4-amino-N-pyrimidin-2-ylbenzenesulfonamide (Sulfadiazine) for 48 hrs, the EC50 was 212mg/l with 95 % CI was 187-240 mg/l. Thus chemical was consider as nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

In the fifth weight of evidence study for the same read across chemical 4-amino-N-pyrimidin-2-ylbenzenesulfonamide (Sulfadiazine) (68-35-9) from Chemosphere 2000. Short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs according to the ISO (1989) and OECD (1996) standard procedures. The daphnia magna was culture in beaker with 20 neonates and maintained in specific test conditions. D. magna was cultured in a fully defined medium M7. Stock solutions were prepared by dissolving the appropriate amount of chemical in M7 medium. The pH of the stock solutions was adjusted to 7.5. The test was performed in static condition with 20 neonates less than 24 hrs. The test containers used were 100-ml glass beakers filled with 25 ml of test solution. The test was performed at 21±0.5°C underdark. The mobility of daphnia was observed at 24 and 48 hrs. After the experiment, the EC 50 value for 4-amino-N-pyrimidin-2-ylbenzenesulfonamide for short term toxicity to aquatic invertebrates was determined to be 221 mg/l. Based on the value, the 4-amino-N-pyrimidin-2-ylbenzenesulfonamide was considered to be not toxic to aquatic invertebrates and can be considered as not classified as per the CLP regulations.

 

Study for the target chemical was supported by the weight of evidence study for the read across chemical (122-11-2) from peer reviewed journal Environment International, 2007. Short term toxicity to aquatic invertebrates was performed in Daphnia magna for 48 hrs at 199.2–296.8 mg/l concentration range. The test was performed in 6 liter of glass jar and Stock solutions were prepared with dimethyl sulfoxide, from which test solutions were prepared with appropriate dilution medium for each test species. After the 48 hrs, the EC 50 value for Sulfadimethoxine (122-11-2) for short term toxicity to aquatic invertebrates was determined to be 248.0 mg/l for 48 hrs. Based on the value, the Sulfadimethoxine (122-11-2) was considered to not toxic to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.

 

On the basis of above results for target chemical N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) (from OECD QSAR toolbox version 3.4, and EPIsuite, 2017) and for its read across substance from peer reviewed journal Chemosphere, 2009, Chemosphere, 2000 and Environment International, 2007 it can be concluded that the test substance N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) is not toxic and can be consider to be not classified as toxic as per the CLP classification criteria. 

 

Toxicity to aquatic algae and cyanobacteria:

Various predicted data for the target compound N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) and supporting weight of evidence studies for its closest read across substane with log Kow as the primary descriptor and on the basis of structural and functional similarity were reviewed for the toxicity on the algae end point which are summarized as below: 

In a prediction done by SSS (2017), Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for N-(benzenesulfonyl) benzenesulfonamide (2618-96-4). The EC50 value was estimated to be 112.831 mg/l when N-(benzenesulfonyl)benzenesulfonamide exposed to Desmodesmus subspicatus for 72hrs. Based on this value it can be concluded that the substance N-(benzenesulfonyl) benzenesulfonamide is considered to be not toxic to aquatic environment as per the criteria mentioned in CLP regulation. 

 

In second prediction using the prediction done by EPI suite, EPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance N-(benzenesulfonyl)benzenesulfonamide (2618 -96 -4). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 390.974 mg/l for green algae for 72 hrs duration. Based on this value, it can be concluded that the test chemical N-(benzenesulfonyl)benzenesulfonamide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 

Study for the target chemical was supported by the weight of evidence study for the read across chemical (88-19-7) from authoritative database j-check, 2017. Study was conducted to determine the toxic nature of chemical 2-methylbenzene-1-sulfonamide on the growth of aquatic green algae for 72hrs. Test conducted under the static system. After the exposure of chemical with the green algae effect was observed at which the algal growth inhibited. Based on the growth rate inhibition the EC50 and NOEC was 170 mg/l and 7.6 mg/l. Based on the areas under the growth curves the EC50 and NOEC was 57, 7.4 mg/l. Based on the EC50 (170 mg/l), it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

On the basis of above results for target chemical N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) (from OECD QSAR toolbox version 3.4, and EPIsuite, 2017) and for its read across substance from authoritative database j-check, 2017 it can be concluded that the test substance N-(benzenesulfonyl)benzenesulfonamide (2618-96-4) is not toxic and can be consider to be not classified as toxic as per the CLP classification criteria. 

Based on the toxicity on both aquatic invertebrates and algae, chemical was concluded as a nontoxic and not classified as per the CLP classification criteria.