1-chlorooctane

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Principles of method if other than guideline:

L 133/118 EG, 1988

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorooctane

EC Number:

203-915-5

EC Name:

1-chlorooctane

Cas Number:

111-85-3

Molecular formula:

C8H17Cl

IUPAC Name:

1-chlorooctane

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

After 3 hours a well-mixed sample was taken of each test batch .

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- activated sludge from a municipal waste water treatment plant
- dry matter: 3.2 g/l resulting in 1.28 g/l in the test replicate

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Hardness:

no data

Test temperature:

20 +/- 2°C

pH:

8.0 - 8.3

Dissolved oxygen:

>2.5 mg/L

Salinity:

no data

Nominal and measured concentrations:

nominal concentrations: 18.0, 45.2, 86.4, 131.2 and 260 mg/L

Details on test conditions:

To different amounts of the slightly soluble test item 100 mL water was added. In parallel 16 mL of synthetic sewage was mixed with 200 mL inoculum and 200 mL drinking water. This mixture was added to the test item in water.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

> 260 mg/L

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 260 mg/L

Effect concentrations 3

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 260 mg/L

Results with reference substance (positive control):

EC10 (3h): 1.3 mg/L
EC50 (3h): 8.1 mg/L

Reported statistics and error estimates:

Linear regression with Probit analysis.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study no inhibition of activated sludge respiration by the test item was observed. The EC50 (3h) of the test item is >260 mg/L.