1-chlorooctane

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorooctane

EC Number:

203-915-5

EC Name:

1-chlorooctane

Cas Number:

111-85-3

Molecular formula:

C8H17Cl

IUPAC Name:

1-chlorooctane

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Stability of the test substance was determined before test start: an aqueous solution with 1 g/l test substance was stirred for 18 h, filtered and the concentration of the test substance was measured after 0h and 72h.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: Chodat
- Source (laboratory, culture collection): originally from Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age of inoculum (at test initiation): 3 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

no data

Test temperature:

23.6 - 24.7°C

pH:

test start: 8.5 -8.6
test end: 9.3 - 9.6

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

nominal: 0.035, 0.063, 0.11, 0.18 and 0.32 mL/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Erlenmeyer flask
- Aeration: sterile
- Light intensity: 8000 Lux
- Initial cells density: 2 x 10E+04/mL
- Control end cells density: 96 x 10E+04/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this aquatic toxicity study with algae the highest concentration obtained under test conditions (saturation) was 0.32 mg/L. No effects were observed within this concentration.