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EC number: 203-103-0 | CAS number: 103-34-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Estrogenic activities of chemicals related to food contact plastics and rubbers tested by the yeast two-hydrid assay.
- Author:
- Ogawa Y, Kawawamura Y, Wakui C, Mutsuga M, Nishimura T, and Tanamoto.
- Year:
- 2 006
- Bibliographic source:
- Food Additives and Contaminants, (23)4:422-430.
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- 4,4'-dithiodimorpholine induced by the S9-mix was tested for its estrogenicity activity using the yeast two-hybrid assay.
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Di(morpholin-4-yl) disulphide
- EC Number:
- 203-103-0
- EC Name:
- Di(morpholin-4-yl) disulphide
- Cas Number:
- 103-34-4
- Molecular formula:
- C8H16N2O2S2
- IUPAC Name:
- 4-(morpholin-4-yldisulfanyl)morpholine
Constituent 1
Test animals
- Details on test animals or test system and environmental conditions:
- no
Administration / exposure
- Route of administration:
- other:
- Vehicle:
- DMSO
- Details on exposure:
- DTDM were dissolved in DMSO at 10^-1 to 10^-5 mol/l (final concentration = 10^-3 to 10^-7 mol/l).
The concentration of DMSO was 1% in the assay, which did not inhibit the yeast growth. - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- no data
- Frequency of treatment:
- no data
- No. of animals per sex per dose:
- no data
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- The yeast two-hybrid cells were preincubated overnight at 30°C with vigorous shaking in a SD medium which was free from tryptophan and leucine. The culture was diluted with 4 volumes of the fresh SD medium and 250 µl of this solution put into a small test tube. The test chemical solution (2.5 µl) was added and incubated for 4h at 30°C. After incubation, 150 µl of the culture solution was placed into each of the 96 wells of a microplate and the absorbancy measured at 595 nm. The rest of the culture was centrifuged at 10,000 rpm for 7 min, alter which the supernatant was removed. The cells were enzymatically digested by incubation with 1 mg/l Zymolyase 20T (200 µl) at 30°C for 15 min. The cell lysate was mixed with 4 mg/ml ONPG (40 µl) and incubated at 30°C for exactly 30 min. The reaction was stopped by the addition of 1 mol/l Na2CO3 (100 µl). After centrifugation at 10,000 rpm for 5 min, the supernatant (150 µl) was placed into each well of a microplate. The absorbances at 420 and 570 nm were read using a microplate reader. The beta-galactosidase activity was expressed as the mean and standard deviation of the results from three separate test tubes.
Preparation of metabolites and their measurement of estrogenic activity :
To a tube containing 990µl of the S9-mix, 10µl of the test chemical solution (mainly 10^-1 to 10-5 mol/l ) was added, incubated at 37°C for 4h and then stored at -80°C until the yeast two-hybrid test was mn as metabolite solution. Each experiment was accompanied by trans-stylbene to confirm the metabolic activity. The yeast two-hybrid cells were pre-incubated overnight at 30°C with vigorous shaking in a SD medium free from tryptophan and leucine, then diluted with 1.5 volumes of fresh 2 x SD medium. In a small test tube, 125 µl of the cell solution and 125µl of the metabolite solution were mixed and then incubated at 30°C for 4h. Thereafter, the same procedure as the Measurement of estrogenic activity by yeast two-hybrid assay was carried out.
Examinations
- Examinations:
- The results were evaluated on the basis of the relative activity, expressed as 10% relative effective concentration (REC10), which is the concentration of the test chemical showing 10% of the agonist activity of 10^-6 mol/l E2, the highest activity level of E2. When the activity of the test chemical was higher than the REC10 within the concentration range tested, the chemical was judged to be positive. When it was judged to be negative, more than the highest dose tested was indicated.
- Positive control:
- 17beta-estradiol (E2)
Results and discussion
- Details on results:
- 4,4'-dithiodimorpholine did not display any estrogenic activity.
Any other information on results incl. tables
Table : Estrogenic activities of DTDM
Compounds |
N°CAS |
REC 10 (mol/l) |
|
Parent compound |
Metabolite |
||
DTDM |
103-34-4 |
> 1.0 x 10^-3 |
> 5.0 x 10^-4 |
E2 (positive control) |
87-18-3 |
3.4 x 10^-10 * |
- |
* = positive result |
Applicant's summary and conclusion
- Conclusions:
- DTDM did not show any estrogenicity.
- Executive summary:
In this study, other chemicals related to food contact plastics and rubbers, and their metabolites induced by the S9-mixture were tested for their estrogenic activities using the yeast two-hybrid assay. DTDM did not show any estrogenicity.
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