Pentasodium bis[5-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-4-hydroxy-3-[(2-hydroxy-5-nitrophenyl)azo]naphthalene-2,7-disulphonato(4-)]chromate(5-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23.11.1987 - 4.12.1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Some parameters such as hardness are not reported. No positive controls are mentioned in report.

Qualifier:

according to guideline

Guideline:

other: internal method A-11 equivalent to OECD203 static

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

10 animals were used per concentration tested.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 °C

pH:

7.8

Dissolved oxygen:

8.0

Details on test conditions:

Two test concentrations 300 mg/l and 1000 mg/l were used. As no mortality was observed, no further concentrations were tested.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

LC50 for FAT 40210/C was considered to be >1000 mg/L.

Executive summary:

FAT 40210/C was tested for acute toxicity to fish in a study conducted using a methodology similar to OECD Guideline 203. The test concentrations of 300 and 1000 mg/L were used. 10 Zebra fish per concentration were tested. No mortality was observed. Hence the LC50 for FAT 40210/C was considered to be >1000 mg/L.

Description of key information

The LC50 of FAT 40210  in this fish acute toxicity test was determined to be >1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

The test substance was tested in two acute toxicity studies in fish at concentrations of 300 and 1000 mg/l. Reactive Black 8 did not show any mortality in zebra fish (brachydanio rerio) at concentrations of 300 mg/l. At 1000 mg/l in one test, some mortality was observed but less than 50 % of tested animals were found dead. In the other study, no mortality was observed at 1000 mg/l concentration. Consequently, the substance is of low toxicological concern to fish and not subject to classification according to CLP (Regulation (EC) No 1272/2008).