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EC number: 412-540-8 | CAS number: 22471-55-2 ET 344 SP
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 April 1993 to 23 April 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Stability of test Concentrations
Verified by chemical analysis of the solvent control and all test groups at 0 and 48 hours. The solvent stock solution used to prepare the test series at 0 hours was also analysed. Data relating to the identity, purity and stability of the supplied test material are the responsibility of the Sponsor. - Vehicle:
- yes
- Details on test solutions:
- Method of Preparation
Preliminary solution in 1% v/v Tween 80-ethanol. 2 g of test material was dissolved in solvent and the volume adjusted to 10 ml to give a 2.00 g/10 ml stock solution. 500 ul of this stock solution was dispersed in aged dechlorinated tap water and the volume adjusted to 5 litres to give a 20 mg/l test concentration from which dilutions were made to give the test series.
Stability of test Concentrations
Verified by chemical analysis of the solvent control and all test groups at 0 and 48 hours. The solvent stock solution used to prepare the test series at 0 hours was also analysed. Data relating to the identity, purity and stability of the supplied test material are the responsibility of the Sponsor. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name Daphnia magna Straus.
Source Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee, (I.R.CH.A), France.
Culture At 21°C in polypropylene vessels containing two litres of aged and dechlorinated tap water.Cultures were fed daily with a suspension of mixed algae (predominantly Chlorella spp.) . Culture conditions ensure that reproduction is byparthenogenesis.
Selection Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Total hardness approximately 50 mg/ L as CaCO3
- Test temperature:
- 21°C
- pH:
- 7.6-7.8
- Dissolved oxygen:
- 7.3-8.3 mg O2/ L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations:0.20, 0.36, 0.64, 1.12, 2.0, 3.6, 6.4, 11.2 and 20 mg/l. These test concentrations were assigned following a preliminary range-finding study where 10 daphnids were exposed for a period of 48 hours to each of the following test concentrations:0.010, 0.10, 1.0, 10 and 20 mg/l. The results showed immobilisation at the test concentrations of 10 and 20 mg/l and no effect at 0.010, 0.10 and 1.0 mg/l indicating the choice of test concentration employed in the test.
Verification of test concentrations showed that at 0 hours the measured test concentrations were all greater than or equal to 80% of nominal value with the exception of the 0.20 mg/l test group which showed a measured concentration of 77% of nominal value. This decline in measured concentration is considered to be caused by losses due to the volatile nature of the test material.
Analysis of the solvent stock solution used to prepare the test series at 0 hours showed the concentration to be near nominal value.
Chemical analysis of the 48-hour old test media showed the measured test concentrations to be in excess of 80% of nominal value with the exception of the 0.20, 0.36 and 0.64 mg/l test groups which showed measured concentrations of 70%, 68% and 78% of nominal value respectively. This decline is considered to be due to the volatile nature of the test material despite carrying out the study in sealed test vessels. - Details on test conditions:
- Exposure Conditions
Test vessels : Glass conical flasks each containing approximately 300 ml test solution to fill the flasks and sealed with ground glass stoppers to reduce evaporation.
Experimental design . 9 test concentrations of 1 control and 1 solvent control (including 100 uL/ L auxiliary solvent), each in duplicate. 20 animals per concentration.
Method of initiation : Daphnia were placed in the test solutions after addition of the test material.
Loading 30 ml test solution per organism.
Photoperiod 16h light : 8h dark.
Temperature . 21 +/- 1°C.
Aeration. None.
Environmental Measurements Temperature was recorded daily. pH and dissolved oxygen concentrations were recorded at initiation and termination of the study. - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- See attached document
- Validity criteria fulfilled:
- yes
- Conclusions:
- "No Observed Effect Concentration" (NOEC) at 48h = 1.12 mg/l
- Executive summary:
TEST SPECIES Daphnia magna Straus tested in the 1st instar.
TEST TYPE Acute toxicity 48h EC50 (immobilisation).
TEST CONDITIONS Static test conditions according to OECD Guide1ine No. 202, referenced as Method C.2 of Commission Directive 84/449/EEC (which constitutes Annex V of Counci1 Directive 67/548/EEC).
Results
The following results are based on nominal test concentration
Duration Endpoint Effect conc. Nominal/Measured Conc. based on Basis for effect Remarks (e.g. 95% CL) 24 h EC50 8 mg/L nominal test mat. mobility 48 h EC50 3.2 mg/L nominal test mat. mobility 48 h NOEC 1.12 mg/L nominal test mat. mobility
Reference
Chemical analysis of the 48-hour old test media showed that at the "No Observed Effect Concentration" at 48 hours and the test concentrations above this level where immobilisation was observed, the measured concentrations were all in excess of 80% of nominal value. Therefore it was considered justifiable to calculate the EC50 values in terms of nominal test concentrations only.
Description of key information
TEST SPECIES Daphnia magna Straus tested in the 1st instar.
TEST TYPE Acute toxicity 48h EC50 (immobilisation).
TEST CONDITIONS Static test conditions according to OECD Guide1ine No. 202, referenced as Method C.2 of Commission Directive 84/449/EEC (which constitutes Annex V of Counci1 Directive 67/548/EEC).
Results
The following results are based on nominal test concentration |
||||||
Duration | Endpoint | Effect conc. | Nominal/Measured | Conc. based on | Basis for effect | Remarks (e.g. 95% CL) |
---|---|---|---|---|---|---|
24 h | EC50 | 8 mg/L | nominal | test mat. | mobility | |
48 h | EC50 | 3.2 mg/L | nominal | test mat. | mobility | |
48 h | NOEC | 1.12 mg/L | nominal | test mat. | mobility |
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.2 mg/L
Additional information
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