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EC number: 412-540-8 | CAS number: 22471-55-2 ET 344 SP
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- The inoculum was prepared by mixing equal volumes of following
three inoculum samples.
1)Filtrate of standard activated sludge
Source ; Chemical Biotesting Center, Chemical Inspection and Testing
Institute, Japan
Date of receipt ; January 21, 1993
2)Effluent from domestic sewage treatment plant
Source ; Research Center, Mitsubishi Kasei Co.
Date of collection ; April 9, 1993
3)River surface water
Source ; Kamoshida River
Date of collection ; April 9, 1993 - Duration of test (contact time):
- 28 d
- Initial conc.:
- 0.92 other: mg
- Based on:
- test mat.
- Details on study design:
- Test bottle :Run #I ; aniline + inoculum + standard dilution water
Run #II ; inoculum + standard dilution water
Run #III ; test substance + inoculum + standard dilution water
Run #IV ; standard dilution water
Run #V ; test substance + water
EXPOSURE
The test was conducted according to the following conditions.
1)Apparatus
Incubator, TAITEC Co., Model LX-30002 )
Condition
Temperature :20 +/- 1°C
Exposure period :28 days (under dark condition)
Test volume :300 mL
Concentration :test substance (Run III and V) ;3.07 mg/L aniline* (Run #I) ;2.00 mg/Lcomposite inoculum (Run #1-III ) ; 100 uL/L(*: Reference substance ; Showa Chemicals, Lot No.SC-3131)
MEASUREMENT OF DISSOLVED OXYGEN CONCENTRATION
The concentration of the dissolved oxygen in the BOD bottle was measured at day 0, 14 and 28.
1)Apparatus : DO meter ; Central Kagaku Co., Model UD-1
DOC MEASUREMENT
The concentration of the DOC was measured as follows.
1)Apparatus : TOC analyzer, Shimadzu Co., model TOC-5000
2)Condition : Furnace temperature : 680°C (TC)
Air flow rate : 150 ml/min.
Sensitivity : X1
Injection volume : 40 ul
3)Calibration curve
The following standard solutions were injected into the TOC analyzer. The calibration curve was prepared by the data processor of the analyzer .
Standard solutions
TC(total carbon) ; 10 mg-C/1 aqueous solution of potassium hydrogen phthalate.
IC(inorganic carbon); 10 mg-C/1 aqueous solution of sodium hydrogencarbonate.
4)Measurement of DOC in the bottles
After measuring dissolved oxygen concentration, 10 ml of the test solution in each bottle was transferred into 10 ml centrifuge tube and centrifuged at 3000 rpm for 10 minutes.
Five ml of supernatant was used for measurement of DOC.
pH MEASUREMENT
After sampling the test solution for DOC measurement, pH of each residual test solution was measured at day 0, 14 and 28.
1)Apparatus : pH meter, Denki Kagaku Keiki, Model COM-10 - Reference substance:
- aniline
- Test performance:
- ACTIVITY OF INOCULUM
Degradability of aniline based on oxygen consumption was 66, 68% at day 28. The activity of the inoculum was judged as satisfactory. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 0
- Sampling time:
- 28 d
- Details on results:
- DEGRADABILITY BASED ON OXYGEN CONSUMPTION
Oxygen consumption* in Run #II was 0.0, -0.1 mg/l, and both 0.1 mg/l in Run #V at day 28. (*:Theoretical value = 8.17 mg)
Degradability based on oxygen consumption were calculated to be 0, -1% i n Run #II, and both 1% i n Run #V.
DEGRADABILITY BASED ON RESIDUAL TEST SUBSTANCE CONCENTRATION
The residual test substance* was detected at concentrations of 2.93 and 2.91 mg/l i n Run #X , and 3.05, 2.93 mg/l in Run #V.
Degradability based on the residual test substance concentration were calculated to be 3, 4% i n Run #X and 2, 5% i n Run #V, respectively.
(*:Theoretical value = 3.07 mg/l)
DEGRADABILITY BASED ON DOC
DOC* i n Run #III was 1.05, 1.03 (as corrected with the average value of Run #II), and 1.59, 1.45 mg/l in Run #V. (*:theoretical value = 2.23 mg/l)
Degradability was not calculated because the test substance was insoluble in water.
pH MEASUREMENT
pH of the test solutions at day 28 were both 7.1 i n Run #X, and were 5.9, 5.8 in Run #V. - Results with reference substance:
- Points of degradation plot based on oxygen consumption:
57 % degradation after 14 d
67 % degradation after 28 d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Based on the results of oxygen consumption, it was concluded that the test substance was not readily biodegradable under the conditions of the Closed Bottle Test.
- Executive summary:
STUDY TITLE
Ready biodegradability test of ET-344-SP
METHOD
The test was conducted according to the method described as "Ready Biodegradability, Closed Bottle Test" in the OECD Guidelines for Testing of Chemicals No.301D.
Test bottle :
Run #I ; aniline + inoculum + standard dilution water
Run #II ; inoculum + standard dilution water
Run #III ; test substance + inoculum + standard dilution water
Run #IV ; standard dilution water
Run #V ; test substance + water
Measurement :
Dissolved oxygen concentration ; at day 0, 14, 28
Residual test substance concentration ; at day 0, 14, 28
Dissolved organic carbon concentration (DOC) ; at day 0, 14, 28
RESULTS
Degradability ( % )
Run# III-1 III-2 V-1 V-2 oxygen consumption 0 -1 1 1 test substance 3 4 2 5 DOC not calculated because test substance was insoluble in water. CONCLUSION
Based on the results of oxygen consumption, it was concluded that the test substance was not readily biodegradable under the conditions of the Closed Bottle Test.
Reference
Description of key information
Based on the results of oxygen consumption, it was concluded that the test substance was not readily biodegradable under the conditions of the Closed Bottle Test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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