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EC number: 203-640-0 | CAS number: 109-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Remarks:
- Test procedure in accordance with national standard methods with acceptable restrictions (e.g. no analytical verification of test item concentrations; test item is assumed to be stable over the exposure period).
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412, part 15
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-Methylmorpholin
- Substance type: pure active substance
- Physical state: liquid, colourless
- Analytical purity: > 99% - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was added to the test water as an aqueous solution (10% w/v), subsequently the fish were added to the test vessel. - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, D-2354 Hohenwestedt, Germany
- Length at study initiation (mean, range): 6.9 cm (6.0 - 7.9 cm)
- Weight at study initiation (mean, range): 2.8 g (1.7 - 4.1 g)
- Feeding during test: withdrawal of food one day before and during the test
ACCLIMATION
- Acclimation period: 2 weeks
- Acclimation conditions: similar, but flow through conditions were used instead of static conditions
- Type and amount of food: "FUKOSALM" - growing feed 1, FUKO - Kraftfutterfabrik, D-7900 Ulm, Germany; amount: ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 2.3 % mortality (medical treatment: 1x 0.05 mg/L malachite green chloride, 1x 10 mg/L tetracycline hydrochloride - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/L
- Test temperature:
- 21 °C
- pH:
- 7.5 to 8.9
- Dissolved oxygen:
- > 6 mg/L
- Nominal and measured concentrations:
- nominal test concentrations: 0.0 (control), 31.6, 46.4, 68.1, 100.0, 147.0, 215.0, 316.0 and 464.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, fill volume: all-glass aquarium; 30 cm x 22 cm x 24 cm; 10 L volume
- Aeration: slight (oil-free air)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.8 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412 part 11, draft Sep 1981. Preparation from fully demineralized tap water
- Metals:
294 .0 mg/l CaCl2 * 2 H20
123 .3 mg/l MgS04 * 7 H20
64 .8 mg/l NaHCO3
5 .8 mg/l KCl
- Ca/Mg ratio: 4:1
- Conductivity: 10 µMHO
- Acid capacity: 0.8 mmol/L
OTHER TEST CONDITIONS
- Photoperiod: 16 h : 8 h day-night-regime
EFFECT PARAMETERS MEASURED:
- mortality and symptoms of distress after 1, 4, 24, 48, 72 and 96 h - pH, oxygen content and temperature at test start and after 24, 48, 72 and 96 h - Reference substance (positive control):
- yes
- Remarks:
- chloracetamide
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 316 - < 464 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 215 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 464 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: gasping and tumbling were observed in the test concentrations above 100 mg/L.
- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- LC50: 48-h LC50: ca. 27 mg/L
- Other: This lethal concentration corresponds to the normal sensitivity. - Sublethal observations / clinical signs:
Cumulative mortality
Concentration
Dead fish (n)
(mg/L)
1h
4h
24h
48h
72h
96h
0.0 (Control)
0
0
0
0
0
0
31.6
0
0
0
0
0
0
46.4
0
0
0
0
0
0
68.1
0
0
0
0
0
0
100.0
0
0
0
0
0
0
147.0
0
0
0
0
0
0
215.0
0
0
0
0
0
0
316.0
0
0
0
1
1
1
464.0
0
2
6
9
10
10
Observed symptoms
Concentration
Observed symptoms
(mg/L)
1h
4h
24h
48h
72h
96h
0.0 (Control)
31.6
46.4
68.1
100.0
147.0
g
g
215.0
g
g
g
g
316.0
g
g
g
g
464.0
g
t
g
g
g = gasping
t = tumbling
pH values
Concentration
pH
(mg/L)
Start
24h
48h
72h
96h
0.0 (Control)
7.8
7.7
7.7
7.7
7.8
31.6
8.2
7.8
7.8
7.8
7.8
46.4
8.3
7.5
7.5
7.5
7.4
68.1
8.2
7.9
7.9
7.9
7.8
100.0
8.3
7.8
7.7
7.8
7.7
147.0
8.6
7.9
7.8
7.8
7.8
215.0
8.7
8.0
7.9
7.9
7.9
316.0
8.8
8.2
7.9
7.8
7.8
464.0
8.9
8.4
8.2
8.2
-
The oxygen content was at least 6.0 mg/L in all test vessels at all time points.
The temperature was reported to be 21 °C throughout the test.
- Conclusions:
- The lowest LC50 value from the range resulting from the key study (316 mg/L) is taken as a worst case approach for further assessment.
Reference
Description of key information
The lowest LC50 value from the range resulting from the key study (316 mg/L) is taken as a worst case approach for further assessment.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 316 mg/L
Additional information
Short-term toxicity of the test substance to fish was investigated by exposing Leusciscus idus (Golden orfe) during 96 hours in a static test, according to the German Industrial Standard DIN 38412, part 15. This resulted in an LC50 value of > 316 and < 464 mg/L, and a NOEC of 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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