Registration Dossier
Registration Dossier
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EC number: 248-096-5 | CAS number: 26896-48-0
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
In a valid study on Soil Microorganisms Nitrogen Transformation according to OECD 216 and EU-method C.21, the following results were obtained for the test item:
NOEC (28 d; nominal): 320 mg/kg soil dw;
EC50 (28 d; nominal): 566 mg/kg soil dw.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 320 mg/kg soil dw
Additional information
For the test item, a Soil Microorganisms Nitrogen Transformation Test according to OECD 216 and EU-method C.21 was performed. Five concentrations were applied ranging from 10 to 1000 mg/kg dry weight. Sieved soil was exposed to the test item for 28 days. On day 0 and after 28 days, the nitrate content in each treatment was determined. A blank control and a solvent control were tested. In the blank control and the solvent control nitrate values at the end of the test were in the same range. An effect of the used solvent on the nitrate production in this test system can be excluded. At the start of the test nitrate concentrations in all test vessels were in the same range. At the end of the test the four lowest treatments did not show any inhibition of nitrate content (rather an increase) compared to the control, whereas the highest treatment 1000 mg/kg showed inhibition of 100 % compared to the control. The relative standard deviation between the replicates of the blank control was 4.8 % and 2.3 % for the solvent control, indicating the small difference between the replicates. All validity criteria were fulfilled and results of the study can be considered reliable.
The following results were obtained:
NOEC (28 d; nominal): 320 mg/kg soil dw;
EC50 (28 d; nominal): 566 mg/kg soil dw.
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