Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

EC50 (48h) = 1.1 mg/L (measured) for the waterflea Daphnia magna (OECD 202)
Toxic effects occur at concentrations above the substances water solubility. The test item was initially supsended in solubilizer. Therefore test concentrations above the solubility in aqua dest could be reached and within the range, water solubility and highest tested concentration, toxic effects were observed .

Key value for chemical safety assessment

Additional information

One study is available on the short-term toxicity of 4,4´-(9H-fluoren-9-ylidene)bis(2-chloroaniline) (CAS No. 107934-68-9) to aquatic invertebrates (Douglas, 1991). The study was performed according to OECD guideline 202 and GLP, with water fleas (Daphnia magna) as test organisms. The daphnids were exposed to the substance for 48 hours at measured test concentrations in the range of 0.083 to 4.5 mg/L (equivalent to nominal concentrations of 0.1 to10 mg/L). Since the substance has a low water solubility (< 0.052 mg/L) the test item was initially suspended in 10% Tween 80. Therefore test concentrations above the solubility in aqua dest could be reached. Up to a measured concentration of 0.8 mg/L no effects on the mobility of the test organisms occurred. At higher concentrations an abrupt increase of immobilized daphnids were determined, which results in an EC50 (48h) of 1.1 mg/L (measured). Especially for filter feeders like Daphnia it is known, that particles can cause secondary effects. Hence, the result of the acute daphnia test was supplemented with long-term investigations on the reproduction of the water fleas.