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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

LC50 (96h) > 6.5 mg/L (measured) for rainbow trout Oncorhynchus mykiss (OECD 203)
No toxic effects occur at the range of the substances water solubility. The test item was initially suspended in solubilizer. Therefore test concentrations above the solubility in aqua dest could be reached and even within these higher tested concentrations no toxic effects occurred.

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Additional information

One study is available on the short-term toxicity of 4,4´-(9H-fluoren-9-ylidene)bis(2-chloroaniline) (CAS No. 107934-68-9) to fishes (Douglas, 1991). The study was performed according to OECD guideline 203 and GLP, with rainbow trout (Oncorhynchus mykiss) as test organism. The fishes were exposed to the substance for 96 hours under semi-static conditions. Since the substance has a low water solubility (< 0.052 mg/L) the test item was initially suspended in 10% Tween 80. The test was conducted as limit test with a measured test substance concentration of 6.5 mg/L (equivalent to a nominal concentration of 10 mg/L). During the 96 hours test duration no effects on the test fishes were observed.