Pentane-2,3-dione

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Desmutagenic effect of α-dicarbonyl and α-hydroxycarbonyl compounds against mutagenic heterocyclic amines

Author:

Kim, S.B., Hayase F, and Kato H.,

Year:

1987

Bibliographic source:

EFSA Journal, 12(11):3888

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

Only TA100 used without metabolic activation

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Pentane-2,3-dione

EC Number:

209-984-8

EC Name:

Pentane-2,3-dione

Cas Number:

600-14-6

Molecular formula:

C5H8O2

IUPAC Name:

pentane-2,3-dione

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
2,3-pentanedione purchased from Nakarai Chemicals Ltd, Kyoto

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 100

Metabolic activation:

without

Test concentrations with justification for top dose:

1.05 µmole
No justification of the top dose provided

Vehicle / solvent:

No information provided

Controls

Positive controls:

yes

Positive control substance:

2-acetylaminofluorene

benzo(a)pyrene

Details on test system and experimental conditions:

Prior to preincubation, 150 µl of each aqueous solution containing 0.37 nmole Trp-P-1, 0.39 nmole Trp-P-2, 0.40 nmole Glu-P-1, 4.53 nmoles Glu-P-2, 0.02
nmole IQ were allowed to react with and without 150 µl of each aqueous solution containing 4.44 µmoles α-hydroxycarbonyls and 1.05 µmoles α-dicarbonyls
at 121°C for 20 min. respectively, and 100 µl of each resulting reaction mixture were used for the mutagenicity test.
The initial pH of the reaction mixture was 7.0.

Rationale for test conditions:

No information provided

Evaluation criteria:

Desmutagenicity was expressed as the ratio of the percentage of the mutagenicity of each mutagenic heterocyclic amine before and after the addition of the test substance.

Statistics:

No information provided

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 100

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

not determinable because of methodological limitations

Applicant's summary and conclusion

Conclusions:

The test chemical was negative for mutagenicity in S typhimurium TA100 without metabolic activation under the conditions of the study.

Executive summary:

The test chemical was tested for mutagenicity in S typhimurium TA100 without metabolic activation. Under the conditions of the study the test substance was negative.