[No public or meaningful name is available]

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 2011 - May 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Cas Number:

890707-29-6

Molecular formula:

C10H11N3O

IUPAC Name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

not specified

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil: 10 g mixture of fine ground quartz sand and 55 mg test substance were mixed with 740 g wet artificial soil for each test container to form 100 mg/kg dry soil test substance systems.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Age at test initiation: 2-3 months
- Weight at test initiation: 308-352 mg

ACCLIMATION
- Acclimation period: 7 d
- Acclimation conditions: same as test
- Health during acclimation: Healthy, no mortality observed

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

17.4-21.1°C

pH:

6.99-7.03

Moisture:

20-30%

Details on test conditions:

TEST SYSTEM
- Test container: Test containers were covered with perforated plastic film to prevent test medium from drying
- Amount of soil or substrate: 740 g wet artificial soil/test container
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition: sphagnum peat 10%, kaolin clay 20%, industrial sand 70%

OTHER TEST CONDITIONS
- Photoperiod: Continuos light
- Light intensity: 400-800 Lux

EFFECT PARAMETERS MEASURED: Mortality observed at day 7 and 14. Total wight of earthworms were determined at beginning and end of test.

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1, 10, 100 and 1000 mg/kg dry soil
- Results used to determine the conditions for the definitive study: The modified mortality in the 7-d range finding study was 5.3%, 0.0%, 0.0% and 15.8% respectively for the test groups.

Nominal and measured concentrations:

Nominal concentration: 100 mg/kg dry soil

Reference substance (positive control):

yes

Remarks:

Chloracetamide

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: No mortality was observed in the control group. Mortality in test substance group (100 mg/kg dry soil): 1/40 earthworms.

Results with reference substance (positive control):

The mortality in the controls did not exceed 10%. The mortality in the test groups (10, 20, 40, 80 and 160 mg/kg) were 5%, 62.5%, 100%, 100% and 100% respectively. The 14d-LC50 of chloracetamide was 18.2 mg/kg dry soil.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the test, the 14d-LC50 of the test substance to Eisenia fetida was greater than 100 mg/kg dry soil

Executive summary:

The acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test according to OECD guideline 207.

The test was conducted as a limit test at a nominal concentration of 100 mg/kg dry artificial soil. The number of mortalities were determined after 7 and 14 days exposure. The mortality of the earthworms in the blank control group was 0% and the test is evaluated as valid.

Under the conditions of the test, the 14d-LC50 of the test substance to Eisenia fetida was greater than 100 mg/kg dry soil.