Onium compounds, morpholinium, 4-ethyl-4-soya alkyl, Et sulfates

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 May 2009 - 30 Jun 2009

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Onium compounds, morpholinium, 4-ethyl-4-soya alkyl, Et sulfates

EC Number:

263-167-0

EC Name:

Onium compounds, morpholinium, 4-ethyl-4-soya alkyl, Et sulfates

Cas Number:

61791-34-2

Molecular formula:

Not available for UVCB substance

IUPAC Name:

4-ethyl-4-(hexadecanoyloxy)morpholin-4-ium 4-ethyl-4-[(9E)-octadec-9-enoyloxy]morpholin-4-ium 4-ethyl-4-[(9E,12E)-octadeca-9,12-dienoyloxy]morpholin-4-ium triethyl sulfate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Cambridge Sewage Treatment Plant, UK
- Pretreatment: Sieved to 850 μm, settled, decanted and resuspended using mineral media. Centrifuged at 4000 rpm for 10 minutes discarding the supernatant, re-suspended and centrifuged again at 4000 rpm for 10 minutes and discharging the supernatant. Dry sludge solids determination was conducted on pellet produced.
- Concentration of sludge: 0.03 g/L (dry sludge solids)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

>= 10 - <= 20 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 22 +/- 2°C
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: conical flasks of 2000 mL volume
- Number of culture flasks/concentration: duplicates
- Method used to create aerobic conditions: : the inoculated mineral medium was aerated by the passage of carbon dioxide free air at a controlled rate.
- Details of trap for CO2: The carbon dioxide is trapped in sodium hydroxide and is measured as dissolved inorganic carbon using a Tekmar-Dohrmann Phoenix 8000. On day 28 1 mL of concentrated HCl is added to each bioreactor, which are then aerated overnight, to drive off the remaining carbon dioxide. One last analysis of evolved carbon dioxide is made on day 29.

SAMPLING
- Sampling frequency: 2, 5, 7, 10, 14, 19, 23, 28, 29 day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, in duplicate
- Reference control: yes, in duplicate
- Toxicity control: yes, single replicate

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

29 d

Remarks on result:

other:

Remarks:

reported as - 7 %

BOD5 / COD results

Results with reference substance:

After 29 days a degradation of 85% was reached.

Any other information on results incl. tables

Table 1: Biodegradation

Time (days)	Biodegradation (%)
	Reference item	Test material	Toxicity control
0	0	0	0
2	28	0	4
5	51	-1	46
7	58	-1	51
10	64	-2	53
14	67	-1	62
19	78	-3	68
23	80	-4	69
28	77	-8	71
29	85	-8	83
29	84	-7	87

Table 2: Validity criteria

Criterion from the guideline	Outcome	Validity criterion fulfilled
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%.	NA no degradation occurred	NA
Percentage degradation of the reference compound has reached the pass levels by day 14.	67%	yes
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d.	62%	yes
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC.	Not reported	Could not be assessed
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium.	33.2 mg/L	yes

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Remarks:

please refer to "any other information on results incl. tables"

Interpretation of results:

not readily biodegradable