Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08/12/2017 to 15/01/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

no

Remarks:

Lab not yet GLP accredited for this end point

Test material

Test material information

Constituent 1

Reference substance name:

Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

EC Number:

261-118-8

EC Name:

Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

Cas Number:

58096-47-2

Molecular formula:

C16H26O2

IUPAC Name:

decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate

Test material form:

liquid

Specific details on test material used for the study:

CAS No: 58096-47-2
EC No: 261-118-8
Chemical Name: Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
Trade Name: Clovanyl-3-Formate (CARYOLAN)
Molecular formula: C16H26O2
Molecular weight: 250.379

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

Preparation of stock solution: 100 mg test sample dissolved in 100 mg of dimethyl formamide and diluted to 100 ml in volumetric flask. (1000ppm)
Preparation of test solution: 0.7 ml pre-culture + 5 ml sample stock solution diluted to 50 ml with test media (Test volume = 50 ml)

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

Not reported

Test temperature:

22 ± 2 ˚C

pH:

initial 6.8 and final 7.0

Dissolved oxygen:

Not reported

Salinity:

Not reported

Conductivity:

Not reported

Nominal and measured concentrations:

100 mg/L test solution

Details on test conditions:

RPM of orbital shaker: 125 rpm
Temperature: 22 ± 2 ˚C
Duration: 72 hours (10.01.2018 to 13.01.2018)
pH: initial 6.8 and final 7.0 .
Pre-culture: Cell concentration 70.8 x 10 4 cells/ml in AAP media.
Vehicle used: Dimethyl formamide (100 ppm)

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

EC50 value for the test substance was found to be >100 ppm.
Growth inhibition in sample + vehicle is negative than growth inhibition in vehicle alone.
As the sample is likely to have low volatility (considering high boiling point and low vapour pressure), this would indicate probably some kind of synergistic effect.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 value for the test substance was found to be >100 ppm.

Executive summary:

Based on the cell counts and percentage growth inhibition of algae, the EC50 value of the test substance as determined by the OECD 201 method was found to be > 100 ppm. This indicates that the chemical is not likely to exhibit aquatic toxicity and hence has not be classified as such.