Reaction mass of Disodium decyl(sulphonatophenoxy)benzenesulphonate and Disodium oxybis[decylbenzenesulphonate]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to guideline and/or standard method but was non-GLP.

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: EPA, 1972. Fish - pesticide acute toxicity test guideline. Pesticides Regulation Division, Environmental Protection Agency.

Deviations:

no

Remarks:

Not specified in report

Principles of method if other than guideline:

Not applicable

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Analytical monitoring:

no

Details on sampling:

No data

Vehicle:

no

Details on test solutions:

All test solutions were prepared in water on a weight-volume basis immediately prior to administering.

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

Source: Zetts Fish Hatcheries, Drifting, Pa.
Date: 5-21-73
Average Weight (g) : 0.85 Range (g) : 0.61-1.41
Average Length (mm) : 38.7 Range (mm) : 34-51
Loading (g/l): 0.49

All test fish were acclimated to laboratory conditions for at least ten days prior to use. Pulverized dry fish pellets were fed to all specimens. Feeding was stopped three days prior to the test to empty the digestive tract. The fish were placed into the bioassay vessels 24 hours before adding the test compound.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

32 mg/L CaCO3

Test temperature:

75F +/- 2F

pH:

7.2-7.6

Dissolved oxygen:

7.8-8.1 mg/L

Salinity:

No data

Nominal and measured concentrations:

Nominal- 0, 4.60, 4.90, 5.24, 5.60, and 6.50 mg/L

Details on test conditions:

Water used in the static test was aerated deionized distilled water with 30 mg/l CaSO4, 30 mg/l MgSO4, 48 mg/l NaHCO3, and 2 mg/l KCL added.

Tests were conducted by placing 16 liters of reconstituted water in each vessel (Glass - 20 liter aquaria), aerating, then adding the fish. Fish loading requirements (<1.0 gm fish / liter water) limited the number of fish to 10 per vessel. If no deaths occurred in 24 hours, aeration was stopped and the test compound added with 1,500 mls of water for mixing, making a total of 17.5 liters. Any vessels containing dead fish were cleaned, reset, and observed for another 24 hours prior to compound addition. Constant temperature water troughs maintained the temperature +/- 2F.

Twenty fish were exposed to each concentration. Observations were made and recorded and dead fish removed daily at the same time for four days (96 hours).

A reference p,p' DDT toxicity was run for both species of fish.

All test solutions were prepared in water on a weight-volume basis immediately prior to administering.

Reference substance (positive control):

yes

Remarks:

p,p' DDT

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.99 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 4.75-5.17

Details on results:

Dowfax 3B-2
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 4.06 3.44-4.40 5.22 4.98-5.42 6.71 6.26-7.71
48 4.10 3.57-4.40 5.06 4.84-5.23 6.24 5.92-6.88
72 4.09 3.56-4.39 5.03 4.81-5.20 6.19 5.88-6.80
96 4.02 3.45-4.34 4.99 4.75-5.17 6.19 5.88-6.84

"Confidence Limits

Controls- No deaths in controls

Results with reference substance (positive control):

p,p' DDT
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 3.4 1.8-4.0 5.1 4.5-5.7 7.7 6.5-13.7
48 3.0 1.3-3.7 4.4 3.4-4.9 6.6 5.9-10.4
72 2.4 0.2-3.4 3.9 1.6-4.5 6.3 5.5-13.9
96 2.0 0.0-3.1 3.5 0.02-4.3 6.2 5.3-141.5

"Confidence Limits

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

yes

Conclusions:

The LC50 at 96 hours for bluegill is 4.99 mg/l.

Executive summary:

The acute fish toxicity of Dowfax 3B-2 was run statically on a cold water species, the rainbow trout, Salmo gairdneri Richardson, and on a warm water representative, the bluegill, Lepomis macrochirus Rafinesque, and via a flow-through system on another species, the fathead minnow, Pimephales promelas Rafinesque.

Using Finney's method of probit analysis, the LC50 at 96 hours for rainbow trout is 5.02 rng/l; for bluegill, 4.99 mg/l; and for fathead minnows, 3.66 mg/l. The higher sensitivity exhibited by the fathead minnows is due primarily to the character of the flow-through test.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to standard method but was non-GLP.

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

no guideline followed

Principles of method if other than guideline:

96-hour flow-through toxicity test in fathead minnows using dechlorinated Lake Huron water

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Analytical monitoring:

no

Details on sampling:

No data

Vehicle:

no

Details on test solutions:

Dechlorinated Lake Huron water was used for the flow-through toxicity with fathead minnows.

The flow-through system was thoroughly cleaned and rinsed with the appropriate toxicant concentration. The toxicant stock solution was made up in water and placed in a Mariotte bottle immediately prior to the test. The system was activated at least three hours before the fish were added so that equilibrium could be reached.

Test organisms (species):

Pimephales promelas

Details on test organisms:

Source: White Bear Lake, Minn.
Date: 2-27-73
Average Weight (g) : 1.25 Range (g) : ,0.59-2.00
Average Length (mni): 49 Range (mm) : 43-60

All test fish were acclimated to laboratory conditions for at least ten days prior to use. Pulverized dry fish pellets were fed to all specimens. Feeding was stopped three days prior to the test to empty the digestive tract. The fish were placed into the bioassay vessels 24 hours before adding the test compound.

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

No data

Test temperature:

54F +/- 2F

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

Nominal- 0, 2.82, 3.75, 5.00 mg/L

Details on test conditions:

Dechlorinated Lake Huron water was used for the flow-through toxicity with fathead minnows. The flow-through system was thoroughly cleaned and rinsed with the appropriate toxicant concentration. The toxicant stock solution was made up in water and placed in a Mariotte bottle immediately prior to the test. The system was activated at least three hours before the fish were added so that equilibrium could be reached.

Ten fathead minnows were placed in each aquarium (glass, 8 liters) with a discharge rate of the diluter being approximately once every eight minutes, or a turnover rate per aquarium of about one/hour. This turnover rate was sufficient to keep ample D.O. A constant temperature water trough maintained the temperature +/-2F. Ten fish were exposed to each concentration.

Reference substance (positive control):

yes

Remarks:

p,p' DDT (acute static)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 3.25-4.11mg/L

Details on results:

Dowfax 3B-2
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 3.15 1.94-3.64 4.26 3.71-5.25 5.76 4.85-11.04
48 3.07 2.22-3.40 3.66 3.25-4.11 4.36 3.94-6.03
72 3.07 2.22-3.40 3.66 3.25-4.11 4.36 3.94-6.03
96 3.07 2.22-3.40 3.66 3.25-4.11 4.36 3.94-6.03
"Confidence Limits

Controls- one control was injured at 24h and died

Results with reference substance (positive control):

p,p' DDT (Acute Static)
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 13.87 --- 18.16 --- 23.76 ---
48 12.60 --- 20.32 --- 32.75 ---
72 12.13 0-13.8 17.47 14.88--- 25.14 18.17---
96 10.05 0-11.86 15.89 13.74-e5 25.13 17.92---

"Confidence Limits

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

yes

Conclusions:

The LC50 at 96 hours for fathead minnow is 3.66 mg/l.

Executive summary:

The acute fish toxicity of Dowfax 3B-2 was run statically on a cold water species, the rainbow trout, Salmo gairdneri Richardson, and on a warm water representative, the bluegill, Lepomis macrochirus Rafinesque, and via a flow-through system on another species, the fathead minnow, Pimephales promelas Rafinesque.

Using Finney's method of probit analysis, the LC50 at 96 hours for rainbow trout is 5.02 rng/l; for bluegill, 4.99 mg/l; and for fathead minnows, 3.66 mg/l. The higher sensitivity exhibited by the fathead minnows is due primarily to the character of the flow-through test.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to guideline and/or standard method but was non-GLP.

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: EPA, 1972. Fish - pesticide acute toxicity test guideline. Pesticides Regulation Division, Environmental Protection Agency.

Deviations:

no

Remarks:

Not specified in report

Principles of method if other than guideline:

Not applicable

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Analytical monitoring:

no

Details on sampling:

No data

Vehicle:

no

Details on test solutions:

All test solutions were prepared in water on a weight-volume basis immediately prior to administering.

Test organisms (species):

other: Salmo gairdneri Richardson

Details on test organisms:

Source: Platte River, Michigan
Date: 10-1-73
Average Weight (g): 1.19 Range ( g ) : 0.95-1.650
Average Length (mm) : 54 Range (mm): 48-59
Loading (g/l) : 0.68

All test fish were acclimated to laboratory conditions for at least ten days prior to use. Pulverized dry fish pellets were fed to all specimens. Feeding was stopped three days prior to the test to empty the digestive tract. The fish were placed into the bioassay vessels 24 hours before adding the test compound.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

32 mg/L CaCO3

Test temperature:

55F +/- 2F

pH:

7.2-7.6

Dissolved oxygen:

7.8-8.1 mg/L

Salinity:

No data

Nominal and measured concentrations:

Nominal- 0, 4.20, 4.60, 5.24, 5.60, 6.50, and 7.50 mg/L

Details on test conditions:

Water used in the static test was aerated deionized distilled water with 30 mg/l CaSO4, 30 mg/l MgSO4, 48 mg/l NaHCO3, and 2 mg/l KCL added.

Tests were conducted by placing 16 liters of reconstituted water in each vessel (Glass - 20 liter aquaria), aerating, then adding the fish. Fish loading requirements (<1.0 gm fish / liter water) limited the number of fish to 10 per vessel. If no deaths occurred in 24 hours, aeration was stopped and the test compound added with 1,500 mls of water for mixing, making a total of 17.5 liters. Any vessels containing dead fish were cleaned, reset, and observed for another 24 hours prior to compound addition. Constant temperature water troughs maintained the temperature +/- 2F.

Twenty fish were exposed to each concentration. Observations were made and recorded and dead fish removed daily at the same time for four days (96 hours).

A reference p,p' DDT toxicity was run for both species of fish.

All test solutions were prepared in water on a weight-volume basis immediately prior to administering.

Reference substance (positive control):

yes

Remarks:

p,p' DDT

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.02 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 4.72-5.28 mg/L

Details on results:

Dowfax 3B-2
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 5.16 4.64-5.44 5.95 5.68-6.33 6.85 6.42-7.96
48 4.87 4.28- 5.21 5.80 5.47-6.14 6.90 6.46-7.82
72 4.43 3.84-4.73 5.17 4.88-5.43 6.02 5.68-6.80
96 4.27 3.65-4.58 5.02 4.72-5.28 5.91 5.57- 6.69

"Confidence Limits

Controls- No deaths in controls

Results with reference substance (positive control):

p,p' DDT
Time LC10 95% LC50 95% LC90 95%
(hrs) C.L. * C.L. * C.L.*
24 9.7 7.2-11.0 13.5 12.1-15.2 18.8 16.4-26.2
48 8.7 6.6-9.9 11.7 10.4-12.8 15.6 14.0-19.6
72 7.6 5.1-9.0 11.0 9.5-12.4 16.1 14.1-21.6
96 5.5 2.3-7.3 9.2 6.7-10.7 15.2 12.9-23.3

"Confidence Limits

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

yes

Conclusions:

The LC50 at 96 hours for rainbow trout is 5.02 mg/l.

Executive summary:

The acute fish toxicity of Dowfax 3B-2 was run statically on a cold water species, the rainbow trout, Salmo gairdneri Richardson, and on a warm water representative, the bluegill, Lepomis macrochirus Rafinesque, and via a flow-through system on another species, the fathead minnow, Pimephales promelas Rafinesque.

Using Finney's method of probit analysis, the LC50 at 96 hours for rainbow trout is 5.02 rng/l; for bluegill, 4.99 mg/l; and for fathead minnows, 3.66 mg/l. The higher sensitivity exhibited by the fathead minnows is due primarily to the character of the flow-through test.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3.66 mg/L

Additional information

There are multiple short-term studies forDOWFAX™ 3B2 with freshwater fish species: Fathead minnow (Pimephales promelas) 96 h LC50 was 1.65 mg/L (a.i.) or 3.66 mg/L formulation;Rainbow trout (Salmo gairdneri) 96 h LC50 was 2.26 mg/L (a.i.) or5.02 mg/L formulation; Bluegill sunfish (Lepomis macrochirus) 96 h LC50 was 2.25 mg/L (a.i.) or 4.99 mg/L formulation.