Methyl 3-methoxypropionate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Recent GLP study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-methoxypropionate

EC Number:

223-358-1

EC Name:

Methyl 3-methoxypropionate

Cas Number:

3852-09-3

Molecular formula:

C5H10O3

IUPAC Name:

methyl 3-methoxypropanoate

Details on test material:

- Lot/batch No.: M50623Y02
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Preliminary range-finding: National Institute of Public Health, 1097 Budapest Gyáli u. 2-6 Hungary, Main: Szent István University, Department of Aquaculture, 2100 Gödöllő, Páter Károly u. 1. – Hungary
- Age at study initiation: < 24 h at beginning of the test
- Feeding during test: no

ACCLIMATION
- Acclimation period: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

control: 20.1-20.3 °C
100 mg/l: 20.1-20.3 °C

pH:

control: 7.30-7.37
100 mg/l: 7.33-7.40

Dissolved oxygen:

control: 8.2-8.3 mg/l
100 mg/l: 8.1-8.1 mg/l

Nominal and measured concentrations:

nominal: 100 mg/l
measured: at the start: 96.7 mg/l, at the end: 82.5 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO medium)

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark

TEST CONCENTRATIONS
- Range finding study: 0.1, 1, 10, 100 mg/l

Reference substance (positive control):

yes

Remarks:

Not concurrent, potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no

Results with reference substance (positive control):

- EC50 (24 h): 1.16 mg/l (95% CL: 1.07-1.25 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

No immobility observed in the control group and dissolved oxygen at the end of the test was > 8 mg/l

Executive summary:

Because no toxic response was observed during the preliminary range-finding test, only a limit test was carried out using only one test concentration (100 mg/l) and one control group in the main study in a static system.

The test concentration was analytically determined at the start and at the end of the experiment. The measured concentration was 96.7 and 82.5 mg/l at the end of the experiment.

No immobility nor abnormal behavior of test animals was observed.