Registration Dossier
Registration Dossier
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EC number: 200-662-2 | CAS number: 67-64-1
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Activated sludge of a predominantly domestic sewage: 30 min EC12=1000 mg/L nominal
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Reliable results were obtained in studies conducted according to guideline and modifications thereof. The results (low toxicity of acetone to microorganisms) are supported by further reliable studies. In a study conducted according to a modification of OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) suitable for testing of highly volatile substances, activated sludge of a predominantly domestic sewage was exposed to acetone under static conditions in closed system. The 30 min EC12 was determined to be 1000 mg/L nominal based on respiration. In a further study conducted according to ISO 8192 (Test for Inhibition of Oxygen Consumption by Activated Sludge) and equivalent or similar to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test), activated sludge was exposed to acetone under static conditions. The 30 min EC50 based on respiration inhibition was determined to be 77.4 mL/L (=61.15 g/L nominal). In further experiments with mixed cultures (75 min BIC=35812 mg/L; 24 h LOEC=10000 mg/L), Pseudomonas putida (16 h NOEC=1700 mg/L), Nitrosomonas (24 h IC50=1200 mg/L), and Paramecium caudatum (4 h LC50=5227 mg/L nominal each) the low toxicity of acetone to microorganisms was confirmed. In a static cell multiplication inhibition test with Entosiphon sulcatum, a 72h-NOEC was determined to be 28 mg/L nominal. This value is regarded as outlier.
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