Acetoacetanilide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Deviation from Guideline (24 instead of 48 h test duration), unclear GLP, rereport from old data

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

The incubation time for daphnia tests in the year 1988 was only 24 hours, not 48 hours as required by OECD 202 (2004). The data on preparing the stock solution and for some test attending measurements are poor

Principles of method if other than guideline:

Incubation time 24 h

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Acetoacetanilide

EC Number:

202-996-4

EC Name:

Acetoacetanilide

Cas Number:

102-01-2

Molecular formula:

C10H11NO2

IUPAC Name:

3-oxo-N-phenylbutanamide

Details on test material:

Acetoacetanilide Batch: L 253

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

DOC

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

24 h

Test conditions

Hardness:

for Breeding and testing the same water quality is used: synthetic water acc DIN 38 412 Part 11

Test temperature:

21°C

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

116 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

other: EC 90

Effect conc.:

1 131 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- EC50/LC50: 0.9 - 1.9 mg/L

Applicant's summary and conclusion

Conclusions:

Under the test conditions the LC50 for Daphnia was determined as 116 mg/L for Acetoacetanilid

Executive summary:

The result of this test gives no reason for classification