Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Lucirin TPO
- Lot/batch No.: 0095, 0096
- Analytical purity: 97.3 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added in the required amount to the test vessel with about 180 ml deionized water. The mixture was stirred for 22 ± 2 hours. Then the inoculum and synthetic medium was added and the test started.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Initial biomass concentration: 1 g/L dry substance

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
180 min

Test conditions

Test temperature:
20 +/- 2 °C
pH:
before correction: 6.7
after correction: 7.2
Nominal and measured concentrations:
Nominal test concentration (mg/l):
1000
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer vessels (nominal 250 mL)
- Fill volume: 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium


Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion