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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Concentration of sludge: 30 mg/l
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: 240 ml inorganic medium, 10 mL inoculum, 100 mg/L test substance
- Test temperature: room temperature
- pH: 7.3 - 7.7
- pH adjusted: no



TEST SYSTEM
- Culturing apparatus: Sapromat
- Number of culture flasks/concentration: 7


SAMPLING
- Sampling frequency: daily


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
0 - 10
Sampling time:
28 d
Details on results:
Kinetic of control substance (in %):
= 70 - 80 after 14 day(s)
Interpretation of results:
other: The test substance is poorly biodegradable and not readily biodegradable according to OECD criteria.

Description of key information

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

The substance was found to be not readily biodegradable in a standard OECD 301F respirometric test. At a test concentration of 100 mg/L the biodegradation degree (BOD of ThOD) was 0 – 10%.