Copper chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Copper monochloride

IUPAC Name:

Copper monochloride

Constituent 2

Reference substance name:

Copper chloride

EC Number:

231-842-9

EC Name:

Copper chloride

Cas Number:

7758-89-6

Molecular formula:

Cl Cu

IUPAC Name:

λ¹-copper(1+) chloride

Details on test material:

Analytical Purity: 97 %
Sigma-Aldrich Corporation, Lot. No. 14014JB

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM

- Length at study initiation: 2.7 ± 0.1 cm
- Weight at study initiation: 0.16 ± 0.01 g
- Feeding during test: No food was
fed before 1 day and during the test period.

ACCLIMATION
- Acclimation period: 7 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

25.4 mg/L as CaCO3

Test temperature:

24.0 ± 0.2 °C

pH:

7.28 - 7.89

Dissolved oxygen:

6.3 - 8.8 mg/L (75 - 105 %)

Nominal and measured concentrations:

Test concentrations were as follows; Nominal concentration: control, solvent control, 0.1, 0.15, 0.24, 0.39, 0.63, 1 mg/L
Mean measured concentration: control, solvent control, 0.021, 0.033, 0.047, 0.047, 0.137, 0.236 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Tap water passed through activated carbon and a membrane filter (1 µm)

OTHER TEST CONDITIONS
- Light intensity: 800 - 1,070 Lux,
- Light periodicity: 16/8 (light/dark)

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.039 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table. Acute toxicity test results for copper monochloride of Oryzias latipes (Unit: mg/L)
Exposure time 24 hrs 48 hrs 72 hrs 96 hrs
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LC50 0.16 0.044 0.039 0.039
LC0 0.047 0.033 0.021 0.021
LC100 - 0.047 0.047 0.047
NOEC 0.033 0.033 0.021 0.021
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