3'-hydroxyacetophenone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Screening Of Tobacco Smoke Constituents for Mutagenicity Using the Ames' Test

Author:

Inger Florin, Lars Rutberg , Margareta Curvall And Curt R. Enzell

Year:

1980

Bibliographic source:

Toxicology, 18 (1980) 219-232

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: refer below principle

Principles of method if other than guideline:

Gene mutation toxicity study was performed to determine the mutagenic nature of the test compound 3'-hydroxyacetophenone

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3'-hydroxyacetophenone

EC Number:

204-494-0

EC Name:

3'-hydroxyacetophenone

Cas Number:

121-71-1

Molecular formula:

C8H8O2

IUPAC Name:

1-(3-hydroxyphenyl)ethan-1-one

Details on test material:

- Name of test material: 3'-hydroxyacetophenone
- Molecular formula: C8H8O2
- Molecular weight: 136.149 g/mol
- Substance type: Organic
- Physical state: No data available
- Purity: No data available
- Impurities (identity and concentrations): No data available

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: Salmonella typhimurium LT-2 strains TA 98 and TA 100

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Liver fraction (S-9) from Aroclor 1254 or methylcholanthrene induced rats

Test concentrations with justification for top dose:

0 or 3 µmole/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: No data available

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: Yes N-methyl-N'-nitro-N-nitrosoguanidin (without metabolic activation) and 2-aminoanthracene (with activation)

Details on test system and experimental conditions:

METHOD OF APPLICATION: Spot test (in agar)

DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: No data available

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER: No data available

Evaluation criteria:

1. Increase in the number of spontaneous revertants
2. The presence of the rfa-mutation was checked by crystal violet inhibition;
3. The presence of the plasmid pKM 101 in strains TA 98 and TA 100 was checked by resistance to ampicillin

Statistics:

No data available

Results and discussion

Test results

Species / strain:

S. typhimurium, other: Salmonella typhimurium LT-2 strains TA 98, TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data available

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results :
negative with and without

The test compound 3'-hydroxyacetophenone is not mutagenic in the bacterium Salmonella typhimurium LT-2 strains TA 98, TA 100 with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Executive summary:

Gene mutation toxicity study was performed to determine the mutagenic nature of the test compound 3'-hydroxyacetophenone.

 

The test material was dissolved in dimethsulphoxide (vehicle control) and applied at a concentration of 3 µmole/plate in the spot test performed.

 

3'-hydroxyacetophenone did not induce reversion of mutant strains and hence is not mutagenic in the bacterium Salmonella typhimurium LT-2 strains TA 98, TA 100 with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.