3'-hydroxyacetophenone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): 1-(3-hydroxyphenyl)ethanone
- Molecular formula: C8H8O2
- Molecular weight: 136.149 g/mol
- Smiles: c1(cc(ccc1)O)C(C)=O
- InChl): -1S/C8H8O2/c1-6(9)7-3-2-4-8(10)5-7/h2-5,10H,1H3
-Substance type: organic
- Physical state: solid

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2.04 cm
- Weight at study initiation (mean and range, SD): average 0.084 g
-Housing: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72 and 96 hrs

Hardness:

185 mg of CaCO3

Test temperature:

23 °C

pH:

-------

Dissolved oxygen:

6.3 - 6.7

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Sublethal observations / clinical signs:

Table 1: Visible Symptoms:

Nominal Concentration (mg/l)	Number of fish	Symptom (cumulated) after
		3 h	6 h	24 h	48 h	72 h	96 h
		V1	V1	V1	V1	V1	V1
Control	8	NS4	NS4	NS4	NS4	NS4	NS4
100	8	NS4	NS4	NS4	M3	M3	M2

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

E: Exopthalamos

SC: Schooling Effect

Table 2: pH

Nominal Concentration (mg/l)		pH readings after
	Number of fish		24 h	48 h	72 h	96 h
			V1	V1	V1	V1
Control	8		7.4	7.5	7.6	7.7
100	8		7.8	7.9	8.0	8.1

Table 3: Oxygen content

Nominal Concentration (mg/l)		Oxygen content (mg/L) after
	Number of fish		24 h	48 h	72 h	96 h
			V1	V1	V1	V1
Control	8		6.7	6.6	6.5	6.4
100	8		6.6	6.5	6.4	6.3

Table 4: Test temperature

Nominal Concentration (mg/l)		Temperature (°C) after
	Number of fish		24 h	48 h	72 h	96 h
			V1	V1	V1	V1
Control	8		23.9	23.8	24.1	23.8
100	8		23.9	23.8	24.1	23.8

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for 1-(3-hydroxyphenyl)ethanone on Zebra Fish Danio rerio was determine to be 100 mg/l.

Executive summary:

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. Test consider to be valid as it fulfilled all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions was maintained throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 78.3 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. Test consider to be valid as it fulfilled all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions was maintained throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 78.3 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. Test consider to be valid as it fulfilled all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions was maintained throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 78.3 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.