3'-hydroxyacetophenone

Endpoint summary

Description of key information

Short term toxicity to fish:

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance 3'-Hydroxyacetophenonea according to OECD Guideline 202. The test substance was tested at the concentrations 0, 4 , 8,16, 32 and 64 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 100 mg/l was prepared by dissolving light beige powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted water. The median effective concentration (EC50) for the test substance 3'-Hydroxyacetophenone, in Daphnia magna was determined to be 22.8 mg/L on the basis of immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrate daphnia magna but as the chemical was readily biodegradable in water hence the chemical 3'-Hydroxyacetophenone was considered to be not classified as per the CLP regulations.

 

Toxicity to algae and cyanobacteria:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3'-Hydroxyacetophenone (CAS no. 121 -71 -1) according to OECD Guideline 201. The stock solution 200mg/L was prepared by dissolving light beige powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 mins. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 12.5, 25, 50, 100 and 200 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 3'-Hydroxyacetophenone in Desmodesmus subspicatus was determined to be 198.4 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical 3'-Hydroxyacetophenone can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Additional information

Summarized results for the toxicity of test chemical 1-(3-hydroxyphenyl)ethanone (CAS No. 121-71-1) on the mortality, mobility and growth rate inhibition of aquatic life’s were studied and mention as follows:

Short term toxicity to fish:

Study was conducted to assess the effect of test chemical 1-(3-hydroxyphenyl)ethanone on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria were loaded with 8 fishes. A static procedure was used for the study. After 96 hours of exposure to test item 1-(3-hydroxyphenyl)ethanone to limit test concentration 100 m/l, LC50 was determine to be 100 mg/l. Based on the LC50 value, it can be consider that the chemical was toxic, but the chemical was readily biodegradable in water thus on that criteria chemical consider to be nontoxic and not classified as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance 3'-Hydroxyacetophenonea according to OECD Guideline 202. The test substance was tested at the concentrations 0, 4 , 8,16, 32 and 64 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 100 mg/l was prepared by dissolving light beige powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted water. The median effective concentration (EC50) for the test substance 3'-Hydroxyacetophenone, in Daphnia magna was determined to be 22.8 mg/L on the basis of immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrate daphnia magna but as the chemical was readily biodegradable in water hence the chemical 3'-Hydroxyacetophenone was considered to be not classified as per the CLP regulations.

 

Toxicity to algae and cyanobacteria:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3'-Hydroxyacetophenone (CAS no. 121 -71 -1) according to OECD Guideline 201. The stock solution 200mg/L was prepared by dissolving light beige powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 mins. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 12.5, 25, 50, 100 and 200 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 3'-Hydroxyacetophenone in Desmodesmus subspicatus was determined to be 198.4 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical 3'-Hydroxyacetophenone can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

 

Thus on the basis of above all effects from various experimental studies, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.