Registration Dossier
Registration Dossier
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EC number: 201-550-6 | CAS number: 84-66-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Microibial inhibition: NOEC 20 mg/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 20 mg/L
Additional information
In accordance with REACH Regulation 1907/2008 Annex VIII Column 2, testing need not be undertaken if microbial toxicity is unlikely (if the substance is insoluble in water or if the substance is readily biodegradable). In a test of ready biodegradability the substance was found to be readily biodegradable. Waiving of the endpoint is thus justified.
A NOEC value has been asssigned based on the concentration used in the ready bidegradability test.
In a review of the aquatic toxicity of eighteen phthalate esters, Staples et.al. (Environmental Toxicology and Chemistry, Vol. 16(5): 875-89l, 1997) summarises published literature on phthalate esters, including diethyl phthalate. No details are available on methodology but the following results are cited:
Test species |
Fresh/salt water |
Measured/nominal concentration |
Test duration |
Endpoint/ criteria |
EC50/LC50 (95%CL) (mg/L) |
NOEC (LOEC) (mg/L) |
Protozoa T. pyriformis |
F |
N |
24 hours |
Growth inhibition |
- |
50 (100) |
Protozoa T. pyriformis |
F |
N |
48 hours |
Growth inhibition |
132 |
50 (100) |
Laboratory digester culture |
F |
M |
5-193 hours |
Anaerobic metabolic inhibition |
- |
100 |
Protozoa Entosiphon sulcatum |
F |
N |
72 hours |
5% inhibition of reproduction |
- |
- (19) |
Bacteria P. phosphoreum |
S |
N |
5-30 minutes |
Light inhibition |
111 |
- |
Bacteria Pseudomonas putida |
F |
N |
16 hours |
3% inhibition of reproduction |
- |
- (400) |
Protozoa Uronema parduczi |
F |
N |
20 hours |
5% inhibition of reproduction |
- |
- (48) |
This supports the view that the substance is not significantly toxic to microorganisms and that the assigned NOEC is conservative.
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