4-benzyloxy-2-nitro-beta-pyrrolidinostyrene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 09-04-2018 to 12-04-2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13/04/2004

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

30/05/2008

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: OECD guidance document no. 23, GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES

Version / remarks:

14/12/2000

Deviations:

not applicable

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Pyrrolidine, 1-[2-[2-nitro-4-(phenylmethoxy)phenyl]ethenyl]-

Cas Number:

99474-22-3

Molecular formula:

C19H20N2O3

IUPAC Name:

Pyrrolidine, 1-[2-[2-nitro-4-(phenylmethoxy)phenyl]ethenyl]-

Sampling and analysis

Analytical monitoring:

yes

Remarks:

The content of the test item in the test solutions was estimated by calculation based on the organic carbon content of the test item and DOC measurement.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.
The husbandry is performed similar to the method described in the OECD guideline.
- Vessels: preserving glasses, nominal volume 2 L
- Medium: M4-Medium (recipe of ELENDT)
- Food: green algae (Desmodesmus subspicatus)
- Medium renewal: twice a week
- Photo period: 16/8 hours, using neon tubes
- Temperature: 20 +/- 2 °C

Study design

Test type:

static

Water media type:

freshwater

Remarks:

ISO test water

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

none

Post exposure observation period:

none

Test conditions

Hardness:

2.502 mg/L

Test temperature:

20 +/- 2°C

pH:

pH ranged from 7.9 to 8.0

Dissolved oxygen:

> 8 mg/L

Salinity:

-

Conductivity:

-

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 2.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: equivalent concentration of test item based on Dissolved Orgnic Carbon determination

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 2.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: equivalent concentration of test item based on Dissolved Organic Carbon determination

Basis for effect:

mobility

Any other information on results incl. tables

Findings

Immobility

Nominal Concentration in mg/L	Immobility24hours					Immobility48hours
	absolute				in %	absolute				in %
Blank control	0	0	0	0	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0

 

pH and O₂

Nominal Concentration in mg/L	pH		O2-Concentration in mg/L
	0 h	48 h	0 h	48 h
Blank control	8.0	7.9	8.5	8.2
100	7.9	7.9	8.4	8.2

 

Analytical determinations

The measured concentration at the beginning of the test was 2 % of the nominal concentration and after 48 hours the measured concentration was 2 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentration.

Since measured concentrations lay in a close range to the blank control, nominal concentrations are also given in the result tables.

The measured concentrations for treatment and blank control are given in the following table:

Nominal Concentration Test Item	Measured TC  t = 0 h	Measured TC  t = 48 h	Measured IC  t = 0 h	Measured IC  t = 48 h
mg/L	mg/L	mg/L	mg/L	mg/L
Blank control	9.84	12.68	9.48	11.06
100	11.48	13.93	9.54	10.89

LOQ (Limit of quantification) TC = 5.43 mg/L

LOQ (Limit of quantification) IC = 2.03 mg/L

Nominal Concentration Test Item	Measured DOC (TC-IC)  t = 0 h	Measured DOC (TC-IC)  t =48 h	Measured DOC minus blank control t = 0 h	Measured DOC minus blank control t = 48 h
mg/L	mg/L	mg/L	mg/L	mg/L
Blank control	0.36	1.61	--	--
100	1.94	3.04	1.58	1.43

 

Nominal Concentration Test Item	Calculated Concentration  Test Item  t = 0 h	Calculated Concentration  Test Item  t = 48 h	% of Nominal concentration t = 0 h	% of Nominal concentration t = 48 h
mg/L	mg/L	mg/L	%	%
Blank control	--	--	--	--
100	2.3	2.0	2	2

 

Nominal Concentration Test Item	Geometric Mean of measured concentrations
mg/L	mg/L
Blank control	--
100	2.1

 

Results

No toxicity occurred no statistical evaluation was conducted.

Parameter	Geometricmean	Nominal
24h EC50	> 2.1 mg/L	> 100 mg/L
48h EC50	> 2.1 mg/L	> 100 mg/L
48h NOEC	≥2.1 mg/L	≥100 mg/L
48h LOEC	> 2.1 mg/L	> 100 mg/L

 

Validity

•            Immobilisation in the controls may not exceed 10 %. Immobilisation in the controls was 0 %.

•            The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.2 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

At the end of the test no toxicity was observed in the treatment and also none of the animals was immobilized in the blank control.

Executive summary:

A GLP-study was perform to asses the short term toxicity of the test item againstDaphnia magnaSTRAUS according to OECD 202 resp. EU C.2. The study was performed as a limit test using one concentration at 100 mg/L. For the test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilized daphnia were counted. At the end of the test no toxicity was observed in the treatment and also none of the animals was immobilized in the blank control.

The following results were determined for the test item (species: Daphnia magna):

-       48h NOEC ≥ 100 mg/L;

-       48h EC50 > 100 mg/L.