(3β,20β)-3-acetoxy-11-oxoolean-12-en-29-oic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13 April 2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(3β,20β)-3-acetoxy-11-oxoolean-12-en-29-oic acid

EC Number:

228-475-1

EC Name:

(3β,20β)-3-acetoxy-11-oxoolean-12-en-29-oic acid

Cas Number:

6277-14-1

Molecular formula:

C32H48O5

IUPAC Name:

(2S,4aS,6aR,6aS,6bR,8aR,10S,12aS,14bR)-10-acetyloxy-2,4a,6a,6b,9,9,12a-heptamethyl-13-oxo-3,4,5,6,6a,7,8,8a,10,11,12,14b-dodecahydro-1H-picene-2-carboxylic acid

Test material form:

solid

Test solutions

Vehicle:

yes

Details on test solutions:

water (ISO medium)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

pt 5.3 descrizione

Study design

Test type:

static

Water media type:

other: reconstitute water (ISO medium according to OECD 202)

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

249 mg/L (as CaCO3)

Test temperature:

Temperature: The test temperature was in the range of 20.9 – 21.2°C measured in the test vessels during the test. The additionally measured ambient temperature within the climate chamber was between 19.9 – 21.8°C

pH:

The pH of the test solutions was not adjusted and did not vary by more than 1.5 units in any one test. The pH was in the range of 7.37 – 8.00 during the test.

Dissolved oxygen:

Oxygen Concentration: The dissolved oxygen concentration was in the range of 6.64– 8.10 mg/L during the test.

Details on test conditions:

Light-Dark Cycle: The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness. The measured light intensity was 723 lux in the climate chamber at the start of the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Immobilization of the test animals (detailed table)

Nominal concentration Replicate Number of Number of immobilised animals
[mg/L] treated animals 24 h 48 h

Control 1 5 0 0
2 5 0 0
3 5 0 0
4 5 0 0
100 1 5 0 0
2 5 0 0
3 5 0 0
4 5 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item Acetylglycyrrhetinic acid/Q had no toxic effects on the mobility of the daphnids up to the limit concentration of 100 mg/L.
All validity criteria were met. The results are based on the nominal test item concentration (100 mg/L) and were determined directly from the raw data.