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EC number: 246-791-8 | CAS number: 25291-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Apr - 27 Jun 2007
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Version / remarks:
- Adopted 14. Jun 1996
- Deviations:
- yes
- Remarks:
- No depuration phase
- Qualifier:
- according to guideline
- Guideline:
- other: “Method for testing the degree of accumulation of chemical substances in fish body“ stipulated in the “Testing for new chemical substances” (November 21, 2003, No. 1121002, Ministry of the Environment, Japan)
- GLP compliance:
- yes
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms: Fish were analysed five times during test duration.
- Sampling intervals/frequency for test medium samples: Test water was analysed once before first analysis of test fish and thereafter.
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods): Control fish were analyzed before the experimental start and after the experimental completion. Four fish were taken at each sampling time and divided into two groups and analysed individually. In addition, six fish were taken out for measurement of lipid content. - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Level 1: 1000 mg/L stock solution was prepared by dissolving the test substance and Tween-80 (10 times amount of it) in N,N-dimethylformamide. 40 mg/L solution of the test item was then prepared from this stock solution by dilution with N,N-dimethylformamide; Level 2: 4 mg/L stock solution of the test item was prepared from stock solution of Level 1 by dilution with N,N-dimethylformamide.
- Controls: Tween 80 was dissolved in N,N-dimethylformamide to prepare a 400 mg/L stock solution as Tween 80 concentration
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80; N,N-dimethylformamide - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: carp
- Source: Karume laboratory chemicals evluation and research institute, Fukuoka, Japan (date of hatch: 3 Sep 2006)
- Age at study initiation (mean and range, SD): yearling fish
- Length at study initiation (length definition, mean, range and SD): 6.8 - 10.9 cm
- Lipid content at test initiation (mean and range, SD): 3.64%
- Health status: Only healthy fish were used for testing.
- Description of housing/holding area: After rearing to test fish size in an aquarium, the fish were transferred to an acclimatizing aquarium and acclimatized after the external disinfection. The fish showing any abnormality were removed and the remainder was reared for 56 d in a flow-through system at 25 +/- 2 °C. Fish were checked for health conditions and transferred to test tanks. Thereafter, the fish were reared at the same temperature in a flow through system for 14 d following external disinfection. The external disinfeciton in the acclimatizing aquarium was carried out in an aqueous solution containing 20 mg/L ELBAZIU and 7 g/L sodium chloride for 24 h. The external disinfection in the test tanks was carried out in an aqueous solution containing 20 mg/L ELBAZIU and 7 g/L sodium chloride for 24 h
- Feeding during test
- Food type: Feed for fry of carp (proteins: ≥ 43%, lipid content: ≥ 3%, manufacturer: Nippon Formula Feed Mfg. C., Ltd., Japan)
- Amount: 2% of total body weight
- Frequency: Twice a day in halves (once a day in all at holiday)
ACCLIMATION
- Acclimation period: 70 d
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Feed for fry of carp (proteins: ≥ 43%, lipid content: ≥ 3%, manufacturer: Nippon Formula Feed Mfg. C., Ltd., Japan)
- Feeding frequency: Twice a day in halved (once a day in all at holiday)
- Health during acclimation (any mortality observed): no; unhealty fish were removed - Route of exposure:
- aqueous
- Justification for method:
- aqueous exposure method used for following reason: The substance has a low potential for adsorption (log Koc 2.65) and a water solubility of 1.21 mg/L. Thus, the aqueos exposure route is considered relevant.
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 60 d
- Total depuration duration:
- 0 d
- Hardness:
- 14.2 mg/L (Ca, Mg)
- Test temperature:
- Level 1: 24.2 - 24.8 °C
Level 2: 24.0 - 24.6 °C
Control: 24.2 - 24.6 °C - pH:
- Level 1: 7.7
Level 2: 7.7 - 8.0
Control: 7.9 - 9.1 - Dissolved oxygen:
- Level 1: 6.9 - 7.3 mg/L
Level 2: 6.9 - 7.3 mg/L
Control: 7.4 - 8.0 mg/L - TOC:
- 0.3 mg/L
- Conductivity:
- 278 µS/cm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 70 L glass tanks for volatile substances
- Renewal rate of test solution (frequency/flow rate): 0.05 mL/min for stock solution and 2000 mL/min for dilution water, 2880 L/day if test water were supplied; Stock solution: 1 L glass bottle cooled in a refrigerator (frequency of renewal: 1-2 times/week)
- No. of organisms per vessel: 47 for Level 1 + 2, 18 for control
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Groundwater from the premises of the laboratory
- Metals: < standard value
- Pesticides: not detected
- Chlorine: not detected
- Alkalinity: 101 mg/L
- Holding medium different from test medium: no
- Intervals of test medium replacement: flow-through
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h light / 10 h dark
- Light intensity: artificial light of white fluorescent lamp - Nominal and measured concentrations:
- Nominal: 1 µg/L (Level 1), 0.1 µg/L (Level 2)
Measured: 0.929 µg/L (average; SD: 0.0482 µg/L, Level 1), 0.0913 µg/L (average; 0.00611 µg/L, Level 2) - Reference substance (positive control):
- no
- Lipid content:
- 3.64 %
- Time point:
- start of exposure
- Remarks on result:
- other: Lipid content was determined with gravimetric analysis after chloroform-methanol extraction (n=6).
- Lipid content:
- 4.83 %
- Time point:
- end of exposure
- Remarks on result:
- other: Lipid content was determined with gravimetric analysis after chloroform-methanol extraction (n=6).
- Conc. / dose:
- 1 µg/L
- Temp.:
- >= 24.2 - <= 24.8 °C
- pH:
- 7.7
- Type:
- BCF
- Value:
- 380 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- steady state
- Conc. / dose:
- 0.1 µg/L
- Temp.:
- >= 24 - <= 24.6 °C
- pH:
- 7.85
- Type:
- BCF
- Value:
- 300 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- steady state
- Remarks on result:
- not measured/tested
- Remarks:
- No depuration time was included in the study.
- Remarks on result:
- not measured/tested
- Remarks:
- No rate constants were calculated.
- Metabolites:
- Since the substance is expected to be stable based on its chemical structure no analysis of possible metabolites was performed.
- Details on results:
- - Behavioural abnormalities: No abnormalities were observed.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to Table 4 ("Any other information on results incl. tables") for detailed information.
Reference
Table 1: Measured concentrations of test item in water [µg/L]
Level |
After 4 d |
After 11 d |
After 24 d |
After 38 d |
After 47 d |
After 60 d |
Average (SD) |
1 |
0.978 |
0.879 |
0.915 |
0.993 |
0.927 |
0.879 |
0.929 (0.0482) |
2 |
0.0954 |
0.0979 |
0.0870 |
0.0696 |
0.0835 |
0.0873 |
0.0913 (0.00611) |
Table 2: Variation of BCFs
Level |
- |
After 38 d |
After 47 d |
After 60 d |
Average of three results |
1 |
Average BCF |
354.94 |
421.30 |
347.42 |
374.55 |
Variation rate from the average of three results (%) |
5.2366 |
12.48 |
72440 |
- |
|
2 |
Average BCF |
329.45 |
285.93 |
282.00 |
299.13 |
Variation rate from the average of three results (%) |
10.137 |
4.4107 |
5.7270 |
- |
Since the variation of BCF values were within ± 20% of the average, it was concluded that a steady state was reached. The BCFss was calculated on the basis of the results presented in Table 2.
Table 3: Measured concentrations of test item in water at the steady state [µg/L]
Level |
After 4 d |
After 11 d |
After 24 d |
Average |
1 |
0.993 |
0.927 |
0.879 |
0.933 |
2 |
0.0969 |
0.0835 |
0.0873 |
0.0892 |
Table 4: Validity criteria
Criterion according to guideline |
Outcome |
Fulfilled? |
The temperature variation is less than ± 2 °C |
Level 1: 24.2 - 24.8 °C Level 2: 24.0 - 24.6 °C Control: 24.2 - 24.6 °C |
Yes |
The concentration of dissolved oxygen does not fall below 60% saturation. |
At least > 80% based on the reported concentrations of dissolved oxygen in test water. |
Yes |
The concentration of the test substance in the chambers is maintained within ± 20% of the mean of the measured values during the uptake phase. |
83.5 - 99.3% |
Yes |
The mortality or other adverse effects/disease in both control and treated fish is less than 10% at the end of the test; where the test is extended over several weeks or months, death or other adverse effects in both sets of fish should be less than 5% per month and not exceed 30% in all. |
No mortality was reported. |
Yes |
Description of key information
BCF: 300 - 380 based on whole body wet weight
Key value for chemical safety assessment
Additional information
One experimental study is available investigating the
bioaccumulation of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene (CAS
25291-17-2) in fish (Daikin Industries, 2007). The study was performed
according to OECD 305 (1996) and GLP with Cyprinus carpio (yearling
fish) as test organism. Fish were exposed via the aqueous phase to 0.1
and 1.0 µg/L for a duration of 60 d without a depuration phase. Aqueous
exposure was considered to be the relevant exposure route since
adsorption of the substance to particulate matter is expected to be low.
Due to volatility of the test item special test vessels were used to
avoid volatilization. Moreover, a flow-through test design with a high
daily renewal rate of 2880 L/d was used in order to maintain test
concentrations constant during exposure. The actual concentrations were
measured several times during exposure and resulted in concentrations
close to nominal (average: 0.929 µg/L, Level 1; 0.0913 µg/L, Level 2).
For preparation of test solutions the solubiliser Tween-80 (10 times) in
N,N-dimethylformamide was used. The concentration of the solubilizing
agent was below 0.1 mg/L as recommended in the guideline.
The steady-state was reached within the duration of the study since
measurements after 38, 47 and 60 d showed that the variation of BCF
values were within ± 20% of the average. The calculated BCF-values were
380 (Level 1, 1 µg/L) and 300 (Level 2, 0.1 µg/L) based on whole body
wet weight. This result indicates a low potential for bioaccumulation
via the aqueous phase.
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