4-[3-(1-naphthylamino)propyl]morpholine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 24, 1998 to May 13, 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: While the study was conducted according to OECD TG 203 and in accordance with the Principles of Good Laboratory practice (GLP), the stability of the test substance under test conditions was not verified analytically.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Minor deviations such as dissolve oxygen concentrations falling below 60% saturation in several test vessels at 48 hours and others which did have an adverse effect on the outcome of the study were noted

Principles of method if other than guideline:

not applicable

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

yes

Details on test solutions:

A stock solution with a nominal concentration of 100000 mg/l was prepared by bringing 5 grams of 4-(3-(1-naphthylamino)propyl)morpholine to a toal volume of 50 ml with dimethylformamide. Appropriate volumes of the stock solution were added to test vessels containing 15 litres of dilution water.

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnow
- Source: commercial supplier (Aquatic Biosystems, Fort Collins, CO)
- Age at study initiation: juvenile
- At the conclusion of the test, the control fish had an average total length of 29.2 mm and an average wet weight of 0.20 grams (loading rate was approximately 0.13 g/l)
- Prior to testing the fish were maintained under flow-through conditions in a 220 litre polyethylene tank
- Feeding during test - Fish were fed dry commercial fish food once per day except during the 48 hours preceding the test and they were not fed during the toxicity test

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): Mortality during the last 48 hours of acclimation was less than 3%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

not applicable

Test temperature:

The temperature ranged from 21.6 - 22.0 °C

pH:

The pH ranged between from 8.0 - 8.5

Dissolved oxygen:

The dissolved oxygen concentration ranged between 4.3 - 9.0 mg/l (mean - 7.8 mg/l)

Salinity:

not applicable

Conductivity:

530 - 620 (no units specified in the report)

Nominal and measured concentrations:

Range finding test - 0 (control and solvent control), 0.10, 1.0, 10 and 20 mg/l (nominal concentrations)
Definitive test - 0 (control and solvent control), 1.3, 2.2, 3.6, 6.0 and 10 mg/l (nominal concentrations)

Details on test conditions:

A range finding test was conducted at 0 (control and solvent control), 0.10, 1.0, 10 and 20 mg/l (nominal concentrations). After 96 hours there was 100% survival in the control, solvent control and at 0.10 and 1.0 mg/l and 100% mortality at 10 and 20 mg/l.
A definitive test was condcuted at 0 (control and solvent control), 1.3, 2.2, 3.6, 6.0 and 10 mg/l (nominal concentrations). The test was terminated after 72 hours because the fish lot number could not be verfified. Thus, this test was determined to be invalid and was repeated.
A final definitive test was condcuted at 0 (control and solvent control - 0.10 ml/l dimethylformaamide), 1.3, 2.2, 3.6, 6.0 and 10 mg/l (nominal concentrations) at 22 ±1 °C. Ten fishes were added to each of the two replicate control and treatment vessels. The test was performed in 20 litre glass aquaria that contained 15 litres of solution (media depth of approximately 19 cm). Test vessels were randomly arranged in a water bath during the 96 hour test and they were loosely covered. A 16:8 hour photopriod cycle was maintained. Gentle aeration was initiated in all test vessels after 48 hours to maintain acceptable dissolved oxygen concentrations.
The number of surviving organisms and the occurence of sublethal effects (immobilization, loss of equilibrium, erratic swimming, loss of reflex, excitability, discolouration and change in behaviour) were determined visually and recorded initially and after 3, 6, 24, 48, 72 and 96 hours. Mortality observations were made twice daily and dead fishes were removed as and when observed. Parameters such as dissolved oxygen, pH, conductivity and temperature were measured and recorded daily in each test chamber. Temperature was continuously monitored.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CI: 4.2 - 5.4 mg/l

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No insoluble material was observed during the definitive test, although test vessels containing 10 mg/l 4-(3-(1-naphthylamino)propyl)morpholine at 72 hrs and all solvent control and treatment test vessels at 96 hours were observed to be slightly cloudy. No mortality was noted in the control and solvent control dose groups. At 3 and 6 hours, surviving fish exposed to 10 mg/l 4-(3-(1-naphthylamino)propyl)morpholine were lying at the bottom of the vessel and exhibited loss of equilibrium. At 24, 48 and 72 hours, fishes exposed to 6.0 mg/l were dark in colour and lethargic. At 48 and 72 hours, fishes exposed to 3.6 mg/l were dark in colour and lethargic. At 96 hours, fishes exposed to 6.0 mg/l were lethargic and lying at the bottom of the vessel. No other sublethal effects were observed during the test.
Under the conditions of the study, exposure of fathead minnows, Pimepales promelas to 4-(3-(1-naphthylamino)propyl)morpholine resulted in the following -
96 hour LC50: 4.8 mg/l (95% CI: 4.2 - 5.4 mg/l) and
96 hour NOEC: 2.2 mg/l

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

The 24 and 48 hour LC50 values were calculated usng the binomical/nonlinear interpolation method and the 72 and 96 hour LC50 values were calculated using the probit method. The no observed effect concentration (NOEC) is the highest concentration of the test substance that allows at least 90% survival of exposed organismsm and does not cause sublethal effects.

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the study, exposure of fathead minnows, Pimepales promelas, to 4-(3-(1-naphthylamino)propyl)morpholine resulted in the following -
96 hour LC50: 4.8 mg/l (95% CI: 4.2 - 5.4 mg/l) and
96 hour NOEC: 2.2 mg/l

Executive summary:

4-(3-(1-naphthylamino)propyl)morpholine was exposed to fathead minnows (Pimephales promelas) for 96 hours according to the OECD TG 203. The test was performed under static conditions with five concentrations, a dilution water control and a solvent control (0.1 ml/L dimethylformamide) at a temperature of 22 ± 1 °C. The reported 96-hour LC50 value was 4.8 mg/L based on nominal concentrations. The 95% confidence intervals for the LC50 were 4.2 – 5.4 mg/L. The 96-hour NOEC value was 2.2 mg/L.

Description of key information

The 96-hour LC50 value of 4.8 mg/L for 4-(3-(1-naphthylamino)propyl)morpholine is based on nominal concentrations of the test material in a static exposure study with fathead minnows (Pimephales promelas).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.8 mg/L

Additional information

4-(3-(1-naphthylamino)propyl)morpholine was exposed to fathead minnows (Pimephales promelas) for 96 hours according to the OECD TG 203.The test was performed under static conditions with five concentrations, a dilution water control and a solvent control (0.1 ml/L dimethylformamide) at a temperature of 22 ± 1 °C. The reported 96-hour LC50 value was 4.8 mg/L based on nominal concentrations. The 95% confidence intervals for the LC50 were 4.2 – 5.4 mg/L. The 96-hour NOEC value was 2.2 mg/L.