4-[3-(1-naphthylamino)propyl]morpholine

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Remarks:

The study described in this report was conducted in a facility which operates in accordance with Good Laboratory Practice principles, however no claim of GLP compliance was intended nor is made for this study.

Type of method:

flask method

Specific details on test material used for the study:

Identification: 4-(3-(1-napthylamino)propyl)morpholine
Appearance/Physical state: brown/black granular solid
Batch: ZA01212016
Purity: 94.4%
Expiry date: 20 January 2019
Storage conditions: room temperature in the dark

Key result

Water solubility:

0.536 g/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

7.47

Preliminary Test Results:

The preliminary estimate of the water solubility at 20.0 ± 0.5 °C was 0.415 g/L. The solution had a pH of 7.82.

Definitive Test Results:

The mean peak areas relating to the standard and sample solutions are shown in the following table:

Solution	Mean Peak Area
Standard 102 mg/L	5.9446 x 10^7
Standard 203 mg/L	1.1737 x 10^8
Standard 203 mg/L	1.0921 x 10^8
Standard 508 mg/L	2.9041 x 10^8
Standard 761 mg/L	3.9823 x 10^8
Standard 1.02 x 10^3mg/L	4.7910 x 10^8
Sample 1A	1.4108 x 10^8
Sample 1B	1.4024 x 10^8
Sample 2A	1.5790 x 10^8
Sample 2 B	1.6024 x 10^8
Sample 3A	1.7239 x 10^8
Sample 3B	1.7271 x 10^8

The concentration (g/L) of test item in the sample solutions is shown in the following table:

Sample Number	Time Shaken at ~ 30 ºC (hours)	Concentration (g/L)	Solution pH
1	24	0.479	7.70
2	48	0.541	7.51
3	72	0.587	7.20

Mean concentration   :          0.536 g/L at 20.0 ± 0.5 ºC
Range                         :          0.479 to 0.587 g/L
Standard Deviation    :          4.83 x 10^-2

Mean pH: 7.47

Validation:

An assessment of the relationship between amount of test item and response gave a curve therefore a quadratic model was used for the calibration. A correlation coefficient (r) of 1.000 was obtained and this was deemed acceptable for the test.

Discussion:

On completion of the equilibration period, the samples contained a large excess test item present at the bottom of the test vessels. After centrifugation, the supernatant was taken for analysis. The supernatant was visually observed to be clear, slightly orange in color and free from undissolved test item. A slight Tyndall beam was noted however this was not considered significant.

The test item consisted of multiple components therefore the total area of all sample peaks were used for the calculation. The profile of the samples differed from that of the standards due to the earlier eluting peaks having higher solubility than the later eluting peaks.

A slight increase in concentration with shaking time was noted, however all results fell within ± 15% as specified by the test guidelines.

Conclusions:

The water solubility of the test item has been determined to be 0.536 g/L of solution at 20.0 ± 0.5 °C.

Executive summary:

Water Solubility. 0.536 g/L of solution at 20.0 ± 0.5 °C, using the flask method, designed to be compatible with Method A.6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

Description of key information

Water Solubility. 0.536 g/L of solution at 20.0 ± 0.5 °C, using the flask method, designed to be compatible with Method A.6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

Key value for chemical safety assessment

Water solubility:

0.536 g/L

at the temperature of:

20 °C

Additional information

The water solubility of the test item has been determined to be 0.536 g/L of solution at 20.0 ± 0.5 °C.