Urea, N,N''-1,4-cyclohexanediylbis-, trans-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD Guideline and following the priciples of Good Laboratory Practice

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea

EC Number:

813-604-1

Cas Number:

68533-01-7

Molecular formula:

C8 H16 N4 O2

IUPAC Name:

[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

NM01; batch no.: 492-14-01
Purity: 99.08 area % (190 nm) and 98.17 area % (200 nm)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

In the definitive test following test item concentrations were used: 31.3, 62.5, 125, 250 and 500 mg/L
plus the control (with a spacing factor of 2.0).

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22.0 – 22.4°C

pH:

The pH values were in the ranges of 7.14 – 7.28 at exposure initiation and 8.11 – 8.58 at exposure termination

Nominal and measured concentrations:

In the definitive test following test item concentrations were used: 31.3, 62.5, 125, 250 and 500 mg/L
plus the control (with a spacing factor of 2.0).

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

The EyC50/72 h is higher than the test item concentration of 500 mg/L.

The EyC10/72 h is 264 mg/L (95% confidence limits were not determined).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes