4-chloro-4'-fluorobutyrophenone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guideline and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-chloro-4'-fluorobutyrophenone

EC Number:

223-395-3

EC Name:

4-chloro-4'-fluorobutyrophenone

Cas Number:

3874-54-2

Molecular formula:

C10H10ClFO

IUPAC Name:

4-chloro-1-(4-fluorophenyl)butan-1-one

Test material form:

other: liquid

Details on test material:

Test item: 4-Chloro-4’-fluorobutyrophenoneCAS number: 3874-54-2EC number: 223-395-3Molecular formula: C10H10ClFOMolecular weight: 200.64 g/molBatch number STBD6247VPhysical state: liquidStorage: cool, dry place in a tightly closed container

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Type of inoculum: aerobic mixed bacterial cultureSource: Municipal wastewater treatment plant, Esztergom-Kertváros

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

PREPARATION OF INOCULUM TEST CULTUREBefore preparation of inoculum test culture, the dry substance content of the stock culture was determined. The measured value was 7.3 g/L. 250.0 ml inoculum test culture with 1.0 g/L dry substance was made by mixing 34 ml of stock culture with 216 ml mineral medium.PREPARATION OF REFERENCE SOLUTIONFor the reference solution 320.13 mg sodium acetate was diluted in 100 ml mineral medium.PREPARATION OF STOCK SOLUTION OF TEST ITEMFor the stock solution 150.06 mg test item was diluted in 1000 ml mineral medium. A homogenous emulsion was prepared by stirring.PREPARATION OF TEST VESSELSFollowing test vessels were prepared and analysed for the study:- Test item (in two replicates)- Blank control (in two replicates)- Reference solution (positive control)- Reference and test item solution (toxicity control)- Sterilized solution of test item (abiotic control)

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

18

Sampling time:

28 d

Remarks on result:

other: mean of two replicates

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

According to OECD guideline the test item should be classified as readily biodegradable if the percentage biodegradation reaches 60% of theoretical oxygen demand within 28 day period in a 10-day window. Based on ready biodegradability testing results the test item 4-Chloro-4’-fluorobutyrophenone is not readily biodegradable.