4-chloro-4'-fluorobutyrophenone

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guideline and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-chloro-4'-fluorobutyrophenone

EC Number:

223-395-3

EC Name:

4-chloro-4'-fluorobutyrophenone

Cas Number:

3874-54-2

Molecular formula:

C10H10ClFO

IUPAC Name:

4-chloro-1-(4-fluorophenyl)butan-1-one

Test material form:

other: liquid

Details on test material:

Test item: 4-Chloro-4’-fluorobutyrophenoneCAS number: 3874-54-2EC number: 223-395-3Molecular formula: C10H10ClFOMolecular weight: 200.64 g/molBatch number STBD6247VPhysical state: liquidStorage: cool, dry place in a tightly closed container

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

The preparation of stock and test solutions was based on the preliminary non GLP range finding test.Seven test solutions of different concentration were prepared by mixing appropriate volume of OECD medium and stock solution of the test item. These concentrations were arranged in a geometric series with a maximum separation factor 2.0.Three additional replicates were prepared for controlling the abiotic degradation of the test substance. These test vessels were not inoculated with alga inoculum culture.The volume of the prepared dilution levels was 300 ml except the control solution which was 600 ml.The alga inoculum culture was diluted 288.46 times so the initial alga cell concentration in every dilution and the control levels were 4 959 cells/ml.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Test species: Pseudokirchneriella subcapitata (Korshikov) F.Hindák (formerly known as Selenastrum capricornutum)Source: UTEX The Culture Collcetion of Algae (USA)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

The temperature inside the incubator at the test vessels was measured and registered continuously. Throughout the test the temperature was nearly constant.

pH:

At the beginning of the test the pH values were measured in the remaining test solution of the concurrent test vessels at each dilution level.At the end of the test the pH values were measured in each test vessel.The measured mean pH values at the beginning of the test varied in the range of 8.03 - 8.15.The measured mean pH values at the end of the test varied in the range of 7.52 - 10.01.

Nominal and measured concentrations:

In the test the following nominal concentrations were applied: 53.34; 26.67; 17.78; 8.89; 7.11; 3.56; and 1.78 mg/L.The following concentrations were calculated from the measured/calculated values in the test: 13.62; 6.58; 4.28; 1.54; 1.47; 0.19 and 0.16 mg test item/L.

Details on test conditions:

Three replicates were prepared at every test and six at the control level. The vessels contained 75 ml test media.The test vessels were closed with silicone stopper and kept in incubator. For the proper mass transfer of carbon dioxide glass tubes with filter were placed trough silicone stoppers. The test was maintained under static conditions for a period of 72 hours. The temperature during the test remained in the 21 ± 2 °C range.The vessels were shaken and illuminated continuously in the incubator. The vessels were placed randomly into the incubator and were repositioned daily after sampling. One of the vessels of abiotic controls was taken into the incubator with a complete aluminium foil cover to check the stability of test item in the absence of light.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

2.5 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % CL

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

2.92 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % CL

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

3.92 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % CL

Details on results:

The ECx for specific growth rate and yield were determined by ToxRat®The values of LOEC and NOEC were also calculated by ToxRat® statistic software but the results were not conclusive or determinable.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to the Council Regulation (EC) 440/2008 4-Chloro-4’-fluorobutyrophenone is hazardous to the aquatic environment and classified into the second acute toxic hazardous category [GHS Acute aquatic hazard Category Acute II. – 72 hr EC50 (for algae or other aquatic plants) > 1 mg/L but ≤ 10 mg/L].