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EC number: 930-690-7
CAS number: -
Table 1: Influence of Shale Oil on oxygen consumption of activated
Nominal Concentration of test chemical (mg/L)
Oxygen consumption rate (mg O2/L min-1)
Mean Oxygen consumption rate for control = 1.051 mg O2/L
min-1(standard deviation = 7.2)
The inhibitory effect of the test item Shale Oil on the respiration rate
of aerobic wastewater microorganisms of activated sludge was
investigated in a 3-hour respiration inhibition test according to the EU
Commission Directive 88/302/EEC, Part C.11 (1988) and the OECD Guideline
for Testing of Chemicals, No. 209 (1984).The activated sludge inoculum
was pre-adapted to the test item at the nominal concentration of 15 mg/L
for two weeks prior to the start of the test. The following nominal
concentrations of the test item were tested: 10, 32, 100, 320 and 1000
The EC50 to activated sludge of shale oil, middle fraction was
determined as 154 mg/L, with 95 % CL of 108 – 222 mg/L.
Based on the rationale for read-across, it is considered acceptable to
use this study to address the same endpoint for the heavy fraction of
The EC50 to activated sludge of shale oil, middle fraction was determined as 154 mg/L, with 95 % CL of 108 – 222 mg/L.
Similar values for short-term toxicity to daphnia were observed between
the middle fraction and heavy fraction of shale oil. This, combined with
analytical data which showed the two fractions to be compositionally
similar, support the validity of a read-across approach to address the
toxicity to microorganisms. Hence, the study conducted on the middle
fraction of shale oil, is considered robust enough to be the key study
for the heavy fraction.
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