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Short-term toxicity to fish

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Description of key information

LC50 (96 h) Zebra fish > 100 mg/l

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Two summaries are available on studies performed according to OECD 203, on the registering substance resulting in LC50 > 1000 mg/L. Even if the reliability has been set as 4, they are consistent, and in agreement with the expected results for the category of Stilbene Fluorescent Whitening Agents and they can support the endpoint as a weight of evidence, together with the results of the most similar substance within the category.

The results for the other substances have not been reported here, but a summary can be found in the data gap of the category, within the Category Justification Report attached in section 13: Eleven over fourteen substances have been tested for Fish acute toxicity and for all of them the LC50 is grather than 100 mg/l

The remaining tests presented as a Weight of evidence have been performed on the analogous dihydroxyethyl derivative tetrasulphonated sodium salt. This substance can be considered the best representative within the category, since it has the same organic functionalities, a different sulphonation degree, but measured water solubility is higher than the analogous hexasulphonated, therefore a higher availability could be expected in the water compartment. The % and chemical identity of organic impurities are also very similar. The category Justification Report is attached in section 13 of this dossier

Acute toxicity for Zebra fish was evaluated in the 96 hours screening test, according to the OECD guideline 203. Two doses were administrated at 562 and 1000 mg/l with no effect.

The study result is supported by other four studies performed between 1973 and 1999 on Zebra fish at 96h for which only a summary is available indicating LC50 all greater than 1000 mg/L

A prolonged semistatic assay over 14 days was conducted with Brachydanio rerio. Endpoints were mortality, signs of intoxication, length and weight. The nominal concentrations were 100, 316 and 1000 mg /L. There was no mortality and no difference in body weight at nominal concentrations of 100 and 316 mg/L. Only at 316 mg/L one fish showed slackening in movement.

Two fish acute toxicity tests are available (Ciba-Geigy, 1994 and 1998) on CAS 16090-02-1 pre-treated with light in order to obtain a high percentage of photodegraded products and verify their impact on aquatic toxicity. Both tests resulted in a LC50 > 100 mg/l, demonstrating that those degradation products are no more dangerous for the environment than the parent compounds.

In conclusion no fish acute toxicity is expected for CAS 68971-49-3 or its degradation products