Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study under GLP conditions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Batch GST 7-18099
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES

- Water solubility (under test conditions): 660mg/l

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
max Age 24 hours

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
18-20°C
pH:
7.7-7.9 pH
Dissolved oxygen:
8.3-8.6 mg/l
Salinity:
hardness 251 mg/l CaCo3 and 2.5 mmol/L Ca/Mg ions proportion 4:1
Details on test conditions:
hardness 251 mg/l CaCo3 and 2.5 mmol/L Ca/Mg ions proportion 4:1
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
30.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confindence
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
11.12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of Pyrazole DHE at 24 h is 30.01 mg/l and 48h 11.12 mg/l.
Executive summary:

The study was perfomed according OECD Guidelinee 202.

The animals were treated and observed for 48 hours. No animal in the control group were immobilisized. The EC50 of Pyrazole DHE at 24 h was 30.01 mg/l and at 48h 11.12 mg/l.