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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin corrosion: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 6 to 7 October, 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP, Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD 431(In Vitro Skin Corrosion: Human Skin Model)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclohexene
EC Number:
203-807-8
EC Name:
Cyclohexene
Cas Number:
110-83-8
Molecular formula:
C6H10
IUPAC Name:
cyclohexene
Details on test material:
Lot No. 10817K
Purity: 99.83%

Test animals

Species:
human

Test system

Type of coverage:
other: in vitro
Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
50 μl of test item was added to each of the three test skin units
Duration of treatment / exposure:
The plates with the treated epidermis units were incubated for the exposure times of 4 hours, 1 hour and 3 minutes at room temperature (18-28°C).

Number of animals:
N/A

Results and discussion

In vitro

Resultsopen allclose all
Irritation / corrosion parameter:
other: other: optical density (OD) at 540nm
Value:
0.054
Remarks on result:
other:
Remarks:
Basis: mean. Time point: 4hr exposure. Max. score: 0.061. (migrated information)
Irritation / corrosion parameter:
other: other: OD at 540nm
Value:
0.253
Remarks on result:
other:
Remarks:
Basis: mean. Time point: 1h exposure. Max. score: 0.291. (migrated information)
Irritation / corrosion parameter:
other: other: OD at 540nm
Value:
0.243
Remarks on result:
other:
Remarks:
Basis: mean. Time point: 3min exposure. Max. score: 0.267. (migrated information)

In vivo

Other effects:
No other observations

Any other information on results incl. tables

The results of the optical density (OD) measured at 540 nm of each extract and the calculated % viability of the cells is presented below:

 Substance  Optical Density(OD)     Valiability(%)
 Cyclohexene, 4hr exposure

 1

  0.058 22.79 
 

 2

 0.044

 18.56
 

 3

 0.061

 25.53
 

 mean

 0.054

 22.29
 Cyclohexene, 1hr exposure  1 0.255   106.88
   2  0.291  121.95
   3  0.213  89.30
   mean  0.253  106.05
 Cyclohexene, 3min exposure  1 0.204   85.26
   2  0.267  111.63
   3  0.258  108.00
   mean  0.243  101.63
 Positive Control(Glacial acetic acid), 4hr exposure  1  0.012  2.09
   2  -0.003 2.09 
   3  0.006 2.09 
   mean  0.005 2.09 
 Negative Control(NaCl(9g/L saline)), 4hr exposure  1  0.187  100
   2 0.289   100
   3 0.240   100
   mean 0.239  100 

Mean Blank OD (4h) = 0.003 units

Mean Blank OD (1h; 3min) = 0.004 units

A significant reduction of cell viability (consistently below the cut off value of 35 %) was detected in the case of all the three tissues after four hours exposure.

No reduction of viability was observed after one hour or three minutes exposure.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information
Conclusions:
In vitro EPISKIN model test with Cyclohexene, the results indicate that the test item is corrosive after 4 hours exposure and not corrosive after1 hour exposure. The test item should be classified as Corrosive.