Cyclohexene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexene

EC Number:

203-807-8

EC Name:

Cyclohexene

Cas Number:

110-83-8

Molecular formula:

C6H10

IUPAC Name:

cyclohexene

Details on test material:

- Analytical purity:98.6wt%
- Impurities (identity and concentrations):0.01wt% BHT(2,6-Di-tert-butyl-4-methylphenol) is included as antioxidant

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source:Charles River Japan
- Age at study initiation:6 weeks
- Weight at study initiation:male 161-181g, female 122-140g
- Fasting period before study:16hr
- Housing:5/cage
- Diet (e.g. ad libitum):ad libitum
- Water (e.g. ad libitum):ad libitum
- Acclimation period:

ENVIRONMENTAL CONDITIONS
- Temperature (°C):23.2-24.4
- Humidity (%):50-64
- Air changes (per hr):20/hr
- Photoperiod (hrs dark / hrs light): 12hr/12hr

IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle:100, 200 and 400mg/mL
- Amount of vehicle (if gavage):0.5mL/100g body weight

MAXIMUM DOSE VOLUME APPLIED:2000mg/kg

Doses:

0 (vehicle), 500, 1000, 2000 mg/kg

No. of animals per sex per dose:

Males, 5; females, 5

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:1, 3, 7 and 14day
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 - < 2 000 mg/kg bw

Mortality:

Each 3 of 5 animals of male and female rats at 2,000 mg/kg b.w. died within 3 days after dosing.

Dose(mg/kg b.w.) 0 500 1000 2000
No.of animals 5 5 5 5
No.of death Male 0 0 0 3
No.of death Female 0 0 0 3

Mortanity Male 0 0 0 60%
Mortanity Female 0 0 0 60%

Clinical signs:

other: Each 3 of 5 animals of male and female rats at 2,000 mg/kg b.w. showed abnormal gait, adoption of a prone position, salivation, piloerection and tremor, and then died within 3 days after dosing. Hypoactivity was observed in all male and female rats given

Gross pathology:

Necropsy of the dead animals revealed pulmonary congestion. Abnormal gait, adoption of a prone position, salivation, piloerection and tremors were also observed in some animals before death.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 values are between 1000 and 2000mg/kg b.w. for both sexes. Therefore, cyclohexene is classified as Category 4 of Acute toxicity (oral) according to CLP(GHS).

Executive summary:

The LD50 values are between 1000 and 2000mg/kg b.w. for both sexes. Therefore, cyclohexene is classified as Category 4 of Acute toxicity (oral) according to CLP(GHS) .