Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-923-5 | CAS number: 4553-62-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study is comparable to OECD 403. Old study, few data but sufficient to conclude that the test substance is toxic by inhalation route.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- : The study was only performed on one sex (male). The hisopathological examination of tissues was not performed.
- GLP compliance:
- no
- Remarks:
- Study performed before GLP statement
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-methylglutaronitrile
- EC Number:
- 224-923-5
- EC Name:
- 2-methylglutaronitrile
- Cas Number:
- 4553-62-2
- Molecular formula:
- C6H8N2
- IUPAC Name:
- 2-methylpentanedinitrile
- Details on test material:
- - Name of test material (as cited in study report): 2-Methylglutaronitrile/ethyl succinonitrile
- Molecular formula (if other than submission substance): no data
- Molecular weight (if other than submission substance): no data
- Smiles notation (if other than submission substance): no data
- InChl (if other than submission substance): no data
- Substance type: no data
- Physical state: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data
- Purity test date: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: ChR-CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: no data
- Age at study initiation: young adult
- Weight at study initiation: 250-296 g
- Fasting period before study: not required
- Housing:no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: no data
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus:no data
- Exposure chamber volume: 20 L
- Method of holding animals in test chamber: no data
- Source and rate of air: no data
- Method of conditioning air: no data
- System of generating particulates/aerosols: no data
- Method of particle size determination: not relevant
- Treatment of exhaust air: no data
- Temperature, humidity, pressure in air chamber: no data
TEST ATMOSPHERE
- Brief description of analytical method used: gas chromatography
- Samples taken from breathing zone: no data
VEHICLE: not relevant
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: no data
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): no data
CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration: no data - Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 4 h
- Concentrations:
- 0.33, 0.34, 0.49, 1.11 mg/L
- No. of animals per sex per dose:
- 6 males/dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight. - Statistics:
- The LC50 was determined according to the statistical method of Finner, DJ (Probit Analysis (1964)).
Results and discussion
- Preliminary study:
- Not applicable
Effect levels
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 0.66 mg/L air
- 95% CL:
- 0.39 - 1.34
- Exp. duration:
- 4 h
- Mortality:
- See Table 7.2.2-1
- Clinical signs:
- other: - At non-lethal levels: Salivation, lacrimation, hyperemia, labored respiration, mild convulsions. - At lethal levels: Severe convulsions, exophthalmos and cyanosis were followed by death immediately to one day post-exposure. This material caused severe c
- Other findings:
- No data
Any other information on results incl. tables
Table 7.2.2 -1: Concentration of MGN (mg/l)/Mortality
Analytical concentration of 2-methylglutaronitrile (mg/L) | Mortality | ||||
0,33 | 2/6 | ||||
0,34 | 0/6 | ||||
0,49 | 3/6 | ||||
1,11 | 5/6 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 based on GHS criteria
- Conclusions:
- Under the test conditions, the LC50/male rats/4 hours for 2-methylglutaronitrile (purity: 85%) was 0.66 mg/L. This material caused severe convulsions prior to death. Therefore, based on this central nervous system effect and LC50 value, 2-methylglutaronitrile/ethylsuccinonitrile (85/14) should be considered in category II (H330: Fatal if inhaled ) according to the CLP regulation 1272/2008 and as toxic by inhalation according to the Directive 67/548/EEC (T, R23).
- Executive summary:
In an acute inhalation toxicity study performed similarly to the OECD test guideline No. 403, six male Albino rats were exposed to 2 -methylglutaronitrile/ehtylsuccinonitrile (85%/14%) heated to 200°C for 4 hours at concentrations of 0.33, 0.34, 0.49 and 1.11 mg/L. Animals were then observed for 14 days. At non-lethal levels, salivation, lacrimation, hyperemia, labored respiration, mild convulsions were observed. At lethal levels, severe convulsions, exophthalmos and cyanosis were followed by death immediately to one day post-exposure.
Inhalation (rat/4h) LC50= 0.66 mg/L (95% confidence limits of 0.39 and 1.34 mg/L).
Under the test conditions, 2 -methylglutaronitrile is considered in Category II (H330: Fatal if inhaled) according to the CLP regulation 1272/2008/EC and as toxic by inhalation according to the directive 67/548/EEC (T, R23).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.