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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

With high probability acutely not harmful to aquatic invertebrates.

Key value for chemical safety assessment

Additional information

One valid study according to guideline methods is available. The acute toxicity of caffeine to the water flea Daphnia magna was investigated in a 48-hour static test according to the guideline 79/831/EWG. The following test concentrations were set up: 0, 3.905, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/L. Four replicates each containing 5 daphnids were prepared per concentration. Test substance concentrations were not verified analytically. Based on nominal concentrations, the 48 hour EC50 was 182 mg/L.