Reaction mass of 5-[{4-[Benzyl(methyl)amino]phenyl}diazenyl]-1,4-dimethyl-4H-1,2,4-triazol-1-ium bromide and 3-[{4-[Benzyl(methyl)amino]phenyl}diazenyl]-1,4-dimethyl-4H-1,2,4-triazol-1-ium bromide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experiment start date - 09 November 1982; Experiment end date - 07 December 1982; Study completion date - 26 July 1983.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Deviations:

yes

Remarks:

Bacteria counted by plating: 4.3 x 10E6 / ml

GLP compliance:

no

Specific details on test material used for the study:

- Test substance identified as: FAT 31016/I
- Appearance: solid
- Storage: room temperature (20-24 °C)

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixture of polyvalent bacteria collected on 9.11.82 from the sewage treatment plant (ARA Rhein)

Duration of test (contact time):

28 d

Initial conc.:

40 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 1 °C
- Lighting: indirect daylight

TEST SYSTEM
- Exposure vessels: 500 ml Erlenmeyer flasks
- Water: According to the specification of the OECD Method 301 A

Reference substance:

other: glucose

Key result

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Results with reference substance:

Biodegradation corrected by the blank control and measured as DOC (mg/L) is for reference substance was calculated to be 94 % on 28th day.

Bacterial activity:

No inhibition of the activity of the bacteria was found.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

FAT 31016/I was concluded to have no biodegradation under test conditions.

Executive summary:

Biodegradation potential of the test substance FAT 31016/I was investigated in a study conducted according to the modified AFNOR test, Guideline OECD 301 A, May 12, 1981. Mixture of polyvalent bacteria collected from the sewage treatment plant (ARA Rhein) was used as test organism for inoculation. The level of biodegradation was calculated as the percentage elimination of the organic carbon. Initial test concentration was determined to be 40 mg/L DOC (nominal). The exposure was carried out for the duration of 28 days. However, 0 % biodegradation was observed for FAT 31016/A, however for the reference substance glucose, the biodegradation was calculated to be 94 %. Hence, FAT 31016/I can be concluded to have no biodegradation under test conditions.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study initiation date - 04 November 1993; Experiment start date - 23 November 1993; Experiment end date - 29 November 1993; Study completion date - 13 December 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)

Deviations:

yes

Remarks:

Aerate over night after addition of the inoculum

GLP compliance:

yes

Specific details on test material used for the study:

- Code number: FAT 31016/P
- En nr.: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of an urban waste water

Duration of test (contact time):

5 d

Initial conc.:

> 6 - < 800 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test concentrations: about 6 to 800 mg test substance / litre dilution water
- Temperature: 20 ± 1 °C
- Duration: 5 days
- Estimation Technique: O2 - Determination measured with an oxygen sensitive electrode system : SYLAND (SAV: 00 107)
- Suppression of nitrification: without suppression of nitrification

Reference substance:

other: Glucose

Remarks:

(Merck Nr. 8337)

Remarks on result:

not measured/tested

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

The BOD5 of the reference substance is: 182 mg O2/I BOD5

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD5 of FAT 31016/P was found to be 0 mg O2/g.

Executive summary:

The biochemical oxygen demand of FAT 31016/P is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test was carried out according to ISO 5815 Second Edition 1989-08-01 (E) corresponding to92/96/EEC C.5. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/I. The BOD5 of FAT- 31016/P was 0 mg O2/g, while the BOD5 of the reference substance was 182 mg O2/l. In another test, the COD of FAT 31061/P was determined to be 1427 mg O2/g. The resultant BOD:COD ratio was found to be zero, indicating the substance is not readily biodegradable.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study initiation date - 11 November 1993; Experiment start date - 18 November 1993; Experiment end date - 19 November 1993; Study completion date - 08 December 1993.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes

Specific details on test material used for the study:

- Code number: FAT 31016/P
- En nr.: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98

Oxygen conditions:

not specified

Inoculum or test system:

other: Titration method

Duration of test (contact time):

2 h

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

- Sample weight: 2.5 mg of the test substance
- Temperature: 148 ± 3 °C
- Duration: 2 hours
- Estimation Technique: Titration (Potentiometric), METROHM Titroprozessor 670 (SAV : 00 111)

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Remarks on result:

not measured/tested

Key result

Parameter:

COD

Value:

1 427 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate (Validity: 200 ± 8 mg O2/I) was 198 mg O2/l.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The COD of FAT 31016/P was 1427 mg O2/g.

Executive summary:

A pre-determinated amount of the test substance FAT - 31016/P dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test substance was tested with a sample weight of 2.5 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. The COD of FAT- 31016/P was 1427 mg O2/g, while that of the reference substance was 198 mg O2/I. In another study, the BOD of FAT 31016/P was determined to be 0 mg O2/g. The resultant BOD:COD ratio was found to be 0, indicating that the substance is not readily biodegradable.

Reference 4

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study initiation date - 04 November 1993; Experiment start date - 10 November 1993; Experiment end date - 02 February 1994; Study completion date - 07 March 1994.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

yes

Remarks:

The sludge concentration in the test was 1040 mg/l instead of 200 -1000 mg/l as recommended in the guidelines.

GLP compliance:

yes

Specific details on test material used for the study:

- Code number: FAT 31016/P
- En nr: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98

Oxygen conditions:

aerobic

Inoculum or test system:

other: Sludge of a biological sewage treatment plant (ARA-Therwil) collected on 04.01.94

Duration of test (contact time):

28 d

Initial conc.:

25 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: 22.0 ± 3 °C (room temperature)
- Test duration: 28 days
- Lighting: indirect daylight
- Estimation Technique: TOC/DOC Analyzer/ SHIMADZU TOC-500
- Amount of substance (conc.): 50 mg/l
- Sludge (conc.): 1040 mg/l

Reference substance:

diethylene glycol

Preliminary study:

Mean value of preliminary tests: 27.0 mg/l

Key result

Parameter:

% degradation (DOC removal)

Value:

55.2

Sampling time:

28 d

Details on results:

Adsorption after 3 hours: 26.7 %

Results with reference substance: The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 98.8 % by 16th day.

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

FAT 31016/P was concluded to be not inherently biodegradable.

Executive summary:

The inherent biodegradability of FAT - 31016/P was determined in a 28 days Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test substance was tested in concentration of 25 mg/l DOC. The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC. The bioelimination of the test substance FAT - 31016/P after 28 days was determined to be 55.2 %, while the bioelimination of the reference substance after 16 days was 98.8 %. Hence, FAT 31016/P was concluded to be not inherently biodegradable.

Description of key information

The substance is not readily biodegradable. It is also not inherently biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater

Additional information

FAT 31016/I was concluded to have no biodegradation under the test conditions in a ready biodegradability test conducted in accordance with AFNOR test, OECD Guideline 301A. The bioelimination of the test substance FAT - 31016/P after 28 days was determined to be 55.2 %, in a modified Zahn-Wellens test conducted according to OECD Guideline 302B. Hence, FAT 31016/P was concluded to be not inherently biodegradable. In another set of studies investigating BOD5 and COD, the BOD5 of FAT 31016/P was determined to be 0 mg O2/g, while the COD was 1427 mg O2/g. Hence, the BOD5/COD equals 0 indicating the substance to be not readily biodegradable. For FAT 31016/I, BOD5/COD ratio was again 0/1440 = 0, further supporting the conclusion that the substance is not readily biodegradable. Hence, taking into account the substance can be concluded to be not readily biodegradable, however, to possess the potential for inherent, primary biodegradability.