Reaction mass of 5-[{4-[Benzyl(methyl)amino]phenyl}diazenyl]-1,4-dimethyl-4H-1,2,4-triazol-1-ium bromide and 3-[{4-[Benzyl(methyl)amino]phenyl}diazenyl]-1,4-dimethyl-4H-1,2,4-triazol-1-ium bromide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experiment start date - 03 January 1983; Experiment end date - 07 January 1983; Study completion date - 23 March 1983.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Oct. 1980

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

- Test Substance: FAT 31016/I
- Appearance: solid
- Storage: room temperature (20 - 24 °C)

Analytical monitoring:

yes

Details on sampling:

Water samples were taken at the begin and the end of the experiment and measured photospectrometrically.

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Weight: 0.29 g
- Length: 29.7 mm
- Age: 4 months
- Acclimation: 25 days in dechlorinated tap water
- Supplier: West-Aquarium, Bad Lauterberg, BRD

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 ± 2 °C

pH:

8.2 - 8.4

Dissolved oxygen:

6.1 - 7.3 O2 (mg/l)

Nominal and measured concentrations:

1.0, 1.78, 3.2, 5.6, 10.0, 17.8 and 32 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 3 litre glass aquaria
- Aeration: slowly aerated
- Size: 10 fish per concentration and control
- Lighting: 12 hours light/12 hours darkness; yellow light (TL 16/40 W)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : pH and O2, every 24 h intervals

TEST CONCENTRATIONS
Range finding study
- Test concentrations: A pretest was carried out at 1, 3, 10, 30, 100 mg/L
- Results used to determine the conditions for the definitive study: In the pretest the approximate LC50 was determined to be 4 ppm.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed with the test concentrations 1.0, 1.78 and 3.2 mg/L. While 10, 30, 90 and 100 % mortality was recorded at the test concentrations 5.6, 10.0, 17.8 and 32 mg/L, respectively.

Reported statistics and error estimates:

LC50 values and confidence limit are calculated with the Probit- Method (according to E. Weber, Grundriss der biologischen Statistik).

Validity criteria fulfilled:

not specified

Conclusions:

The 96 h LC50 was calculated to be 10.8 mg/L.

Executive summary:

A study was conducted to evaluate the short term toxicity potential of FAT 31016/I to fish. The study was performed in accordance with the OECD Test guideline No. 203 (Oct. 1980). Based on the results obtained in the pretest the test concentrations were chosen as follows: 1,0; 1,78; 3,2; 5,6; 10; 17,8; 32 mg/L and control. 10 fish each were exposed at each concentration and observed for 96 hours. No mortality was observed with the test concentrations 1.0, 1.78 and 3.2 mg/L. While 10, 30, 90 and 100 % mortality was recorded at the test concentrations 5.6, 10.0, 17.8 and 32 mg/L, respectively. Based on the findings of this study, the LC50 was calculated to be 10.8 mg/L.

Description of key information

The LC50 to fish was determined to be 10.8 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10.8 mg/L

Additional information

A study was conducted to evaluate the short term toxicity potential of FAT 31016/I to fish. The study was performed in accordance with the OECD Test guideline No. 203 (Oct. 1980). Based on the results obtained in the pretest the test concentrations were chosen as follows: 1,0; 1,78; 3,2; 5,6; 10; 17,8; 32 mg/L and control. 10 fish each were exposed at each concentration and observed for 96 hours. No mortality was observed with the test concentrations 1.0, 1.78 and 3.2 mg/L. While 10, 30, 90 and 100% mortality was recorded at the test concentrations 5.6, 10.0, 17.8 and 32 mg/L, respectively. Based on the findings of this study, the LC50 was calculated to be 10.8 mg/L. In another supporting study, the LC50 was determined to be 40 mg/L.