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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 2014 - November 2014
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Barium oxide, obtained by calcining witherite
EC Number:
215-127-9
EC Name:
Barium oxide, obtained by calcining witherite
Cas Number:
1304-28-5
Molecular formula:
Ba O
IUPAC Name:
oxobarium
Details on test material:
Batch identification: MKBR7485V
Barium: 88.7 g/ 100 g

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Origin: aeration tankof the wastewater treatment plant of Mannheim, Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
19.9 - 20.5 °C
pH:
8.7 - 12.3 Measured before correction)
7.2 - 7.5 (corrected before addition of inocolum)
7.9 - 8.1 (after incubation time
Dissolved oxygen:
> 7 mg/L (immediately before measurements)
Nominal and measured concentrations:
Nominal concentrations: 1000 mg/L, 500 mg/l, 250 mg/L, 125 mg/L, 62.5 mg/L
Details on test conditions:
Test replicates: 6 replicates for the control,
3 replicates for each test substance concentration,
2 replicates for each reference substance concentration.
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: respiration inhibition
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: respiration inhibition
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: repiration inhibition
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: respiration inhibition
Results with reference substance (positive control):
The EC50 of 3,5-dichlorophenol was 8.5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes