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EC number: 211-103-7 | CAS number: 629-70-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Nominal test concentrations gave a 48-hour EC50 value of greater than 100 % v/v saturated solution. The NOEC was found to be 100 % v/v saturated solution. The study showed that there were no toxic effects at saturation.
Key value for chemical safety assessment
Additional information
GUIDELINE
A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202 "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
METHODS
Preliminary solubility work indicated that it was not possible to obtain a testable solution of the test item using traditional methods of preparation such as ultrasonication and high shear mixing.
A preliminary media preparation trial indicated a saturated solution method of preparation was most appropriate for the test item.
Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at a concentration of 100 % v/v saturated solution for 48 hours at a temperature of approximately 21 °C under static test conditions. The test item solution was prepared by stirring an excess (100 mg/L) of test item in test water using a propeller stirrer at approximately 1500 rpm for 48 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Sartorius Sartopore filter, first approximate 2 L discarded in order to pre-condition the filter) to produce a 100 % v/v saturated solution of the test item.
RESULTS
Chemical analysis of the test preparations at 0 and 48 hours showed measured test concentrations of less than 0.0062 mg/L, which had been determined to be the limit of quantification (LOQ). This does not infer that no test item was in solution, just that any dissolved test item was a concentration of less than the LOQ.
Exposure of Daphnia magna to the test item gave EC50 values based on the nominal test concentrations of greater than 100 % v/v saturated solution. The No Observed Effect Concentration was 100 % v/v saturated solution.
CONCLUSION
The study showed that there were no toxic effects at saturation.
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