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EC number: 200-607-2 | CAS number: 65-31-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- for read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity study of fish for the test chemical :
2.Study was conducted to investigate the effect of test chemical Nicotine sulfate on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs.
3.Effect of test material was observed on Oncorhynchus mykiss - GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- 2.Chemical analyzed with a Hewlett-Packard 5840A gas chromatograph equipped with model 7671 A auto injector and also with FID.
- Vehicle:
- not specified
- Details on test solutions:
- 2. test material was extracted with the methylene chloride with the additions of saturated sodium borate buffer solution
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- 2. Details on test organisms
Test organism Oncorhynchus mykiss
- Common name: Rainbow Trout
- Source: Test organisms obtain from the stock-culture units of the Environmental Research Laboratory-Duluth
- Weight at study initiation (mean and range, SD): 12.8 grams
- Feeding during test: Not fed 24 hrs before and during the test
Details on test organisms
Test organism Lepomis macrochirus
- Common name: Bluegill
- Source: Test organisms obtain from the stock-culture units of the Environmental Research Laboratory-Duluth
- Weight at study initiation (mean and range, SD): 0.5 g
- Feeding during test: Not fed 24 hrs before and during the test - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 2) 44.7 (40.8 to 47.6 mg/L CaCO3)
- Test temperature:
- 2) 17.2 ° C (16.5 to 18.4 ° C)
3) 12 ° C - pH:
- 2) 7.39 (6.84 to 7.80)
- Dissolved oxygen:
- 2) 8.7 (4.8 to 10.7 mg/L)
- Details on test conditions:
- 2) Details on test conditions
TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top
- Renewal rate of test solution (frequency/flow rate): Flow-through
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water obtain from Lake superior. 2 L proportional diluter with a dilution factor of 0.5 was used.
- Alkalinity: 43.0 mg/L CaCO3 (40.4 to 49.5 mg/L CaCO3)
OTHER TEST CONDITIONS
- Adjustment of pH: Yes
- Photoperiod: 12 hrs photoperiod was used
- Light intensity: 17 lumens at the surface of water.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and all other parameters including pH, temperature were measured
- Results used to determine the conditions for the definitive study: Mortality - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 7.31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % CI was 6.29- 8.50 mg/l
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Conclusions:
- The test chemical is likely to be toxic to fish in the dose range of 4 - 7.31 mg/l
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:
Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.
The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.
The test chemical is likely to be toxic to fish in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.
Reference
Description of key information
Short-term toxicity to fish:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:
Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.
The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.
The test chemical is likely to be toxic to fish in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 7.31 mg/L
Additional information
Short-term toxicity to fish:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:
Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.
The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.
The test chemical is likely to be toxic to fish in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.
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