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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
for read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of fish for the test chemical :
2.Study was conducted to investigate the effect of test chemical Nicotine sulfate on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs.

3.Effect of test material was observed on Oncorhynchus mykiss
GLP compliance:
not specified
Analytical monitoring:
yes
Details on sampling:
2.Chemical analyzed with a Hewlett-Packard 5840A gas chromatograph equipped with model 7671 A auto injector and also with FID.
Vehicle:
not specified
Details on test solutions:
2. test material was extracted with the methylene chloride with the additions of saturated sodium borate buffer solution
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
2. Details on test organisms
Test organism Oncorhynchus mykiss
- Common name: Rainbow Trout
- Source: Test organisms obtain from the stock-culture units of the Environmental Research Laboratory-Duluth
- Weight at study initiation (mean and range, SD): 12.8 grams
- Feeding during test: Not fed 24 hrs before and during the test

Details on test organisms
Test organism Lepomis macrochirus
- Common name: Bluegill
- Source: Test organisms obtain from the stock-culture units of the Environmental Research Laboratory-Duluth
- Weight at study initiation (mean and range, SD): 0.5 g
- Feeding during test: Not fed 24 hrs before and during the test
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
2) 44.7 (40.8 to 47.6 mg/L CaCO3)
Test temperature:
2) 17.2 ° C (16.5 to 18.4 ° C)
3) 12 ° C
pH:
2) 7.39 (6.84 to 7.80)
Dissolved oxygen:
2) 8.7 (4.8 to 10.7 mg/L)
Details on test conditions:
2) Details on test conditions
TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top
- Renewal rate of test solution (frequency/flow rate): Flow-through
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2




TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water obtain from Lake superior. 2 L proportional diluter with a dilution factor of 0.5 was used.
- Alkalinity: 43.0 mg/L CaCO3 (40.4 to 49.5 mg/L CaCO3)

OTHER TEST CONDITIONS
- Adjustment of pH: Yes
- Photoperiod: 12 hrs photoperiod was used
- Light intensity: 17 lumens at the surface of water.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and all other parameters including pH, temperature were measured


- Results used to determine the conditions for the definitive study: Mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.31 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95 % CI was 6.29- 8.50 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality
Conclusions:
The test chemical is likely to be toxic to fish in the dose range of 4 - 7.31 mg/l
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:

Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.

The test chemical  is  likely to be toxic to fish  in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:

Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.

The test chemical  is  likely to be toxic to fish  in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
7.31 mg/L

Additional information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical.The studies are as mentioned below:

Study was conducted to investigate the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill) by providing the exposure period of 96hrs. According to the OECD guideline 202 test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline.Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID.Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms not fed 24 hrs before and during the test.Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top.Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used.As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Oncorhynchus mykiss (Rainbow Trout) by the exposure of Nicotine sulfate for 96 hrs, the LC50 was determine to be 7.31 mg/l. And based on the mortality of Lepomis macrochirus (Bluegill), the LC50 was determine to be 4.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.

The above information was further supported by data for structurally similar read across, short term 96 h test was conducted to evaluate effect of test material on Oncorhynchus mykiss (Rainbow trout, age 13 -21 days fry) . The median lethal effect concentrtaion LC50 was observed to be 4 mg/l . Based on the effect concentration it can be concluded that test material is toxic to fish an d can be classified as aquatic chronic 2.

The test chemical  is  likely to be toxic to fish  in the dose range of 4 - 7.31 mg/l.Based on the LC50 chemical was consider as toxic and classified as chronic 2 as per the CLP classification criteria.